Podocalyxin-Like Protein 1 Regulates TAZ Signaling and Stemness Properties in Colon Cancer
Abstract
:1. Introduction
2. Results
2.1. Expression of Podocalyxin-Like Protein 1 (PODXL) Is Associated with Transcriptional Coactivator with PDZ-Binding Motif (TAZ) Downstream Signaling and Poor Survival in Colon Cancer
2.2. PODXL Regulates TAZ and Downstream Gene Expressions
2.3. Expression of PODXL Is Associated with the Stem Cell Signature in Colon Cancer
2.4. PODXL Regulates Epithelial-Mesenchymal Transition (EMT) Gene Expressions and Aggressiveness in Colon Cancer
2.5. PODXL Confers Resistance to Conventional Chemotherapies
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Chemicals
4.2. Cell Viability and Colony Formation
4.3. Plasmid Transfection
4.4. Real-Time PCR
4.5. Transwell Migration and Invasion
4.6. Tumorsphere Formation Assay
4.7. Western Blot Analysis
4.8. Database Analysis
4.9. Statistical Analyses
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of interest
References
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Lee, W.-Y.; Kuo, C.-C.; Lin, B.-X.; Cheng, C.-H.; Chen, K.-C.; Lin, C.-W. Podocalyxin-Like Protein 1 Regulates TAZ Signaling and Stemness Properties in Colon Cancer. Int. J. Mol. Sci. 2017, 18, 2047. https://doi.org/10.3390/ijms18102047
Lee W-Y, Kuo C-C, Lin B-X, Cheng C-H, Chen K-C, Lin C-W. Podocalyxin-Like Protein 1 Regulates TAZ Signaling and Stemness Properties in Colon Cancer. International Journal of Molecular Sciences. 2017; 18(10):2047. https://doi.org/10.3390/ijms18102047
Chicago/Turabian StyleLee, Wen-Ying, Chih-Chia Kuo, Bo-Xing Lin, Chia-Hsiung Cheng, Ku-Chung Chen, and Cheng-Wei Lin. 2017. "Podocalyxin-Like Protein 1 Regulates TAZ Signaling and Stemness Properties in Colon Cancer" International Journal of Molecular Sciences 18, no. 10: 2047. https://doi.org/10.3390/ijms18102047