CD11c+ CD8+ T Cells Reduce Renal Fibrosis Following Ureteric Obstruction by Inducing Fibroblast Apoptosis
Abstract
:1. Introduction
2. Results
2.1. CD8 Deficiency Promotes Renal Fibrosis in Unilateral Ureteric Obstruction (UUO) Mouse Model
2.2. Activation of CD8+ T Cell Requires an Inflammatory Microenvironment
2.3. CD8 Deficiency Reduces Cytotoxicity and Cell Apoptosis in Obstructed Kidney
2.4. CD11c+ CD8+ T Cell Induces Fibroblast Death
3. Discussion
4. Materials and Methods
4.1. Animals and Surgery
4.2. Histology and Imaging
4.3. RT-PCR Analysis
4.4. Tissue Preparation, Fluorescence-Activated Cell Sorting, and Flow Cytometry
4.5. Fibroblast Isolation and Culture
4.6. Co-Culture Experiments
4.7. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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mRNA | Forward | Reverse |
---|---|---|
PRF-1 | 5′-AAAAACTCCCTAATGAGAGACGC-3′ | 5′-ACACGCCAGTCGTTATTGATATT-3′ |
GZMA | 5′-TGCTGCCCACTGTAACGTG-3′ | 5′-GGTAGGTGAAGGATAGCCACAT-3′ |
GZMB | 5′-CCACTCTCGACCCTACATGG-3′ | 5′-GGCCCCCAAAGTGACATTTATT-3′ |
FasL | 5′-TCCGTGAGTTCACCAACCAAA-3′ | 5′-GGGGGTTCCCTGTTAAATGGG-3′ |
CCL2 | 5′-GTCTGTGCTGACCCCAAGAAG-3′ | 5′-TGGTTCCGATCCAGGTTTTTA-3′ |
CCL3 | 5′-TTCTCTGTACCATGACACTCTGC-3′ | 5′-CGTGGAATCTTCCGGCTGTAG-3′ |
CCL4 | 5′-TTCCTGCTGTTTCTCTTACACCT-3′ | 5′-CTGTCTGCCTCTTTTGGTCAG-3′ |
CCL5 | 5′-AGATCTCTGCAGCTGCCCTCA-5′ | 5′-GGAGCACTTGCTGCTGGTGTAG-3′ |
GAPDH | 5′-TGCCCCCATGTTTGTGATG-3′ | 5′-TGTGGTCATGAGCCCTTCC-3′ |
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Wang, H.; Wang, J.; Bai, Y.; Li, J.; Li, L.; Dong, Y. CD11c+ CD8+ T Cells Reduce Renal Fibrosis Following Ureteric Obstruction by Inducing Fibroblast Apoptosis. Int. J. Mol. Sci. 2017, 18, 1. https://doi.org/10.3390/ijms18010001
Wang H, Wang J, Bai Y, Li J, Li L, Dong Y. CD11c+ CD8+ T Cells Reduce Renal Fibrosis Following Ureteric Obstruction by Inducing Fibroblast Apoptosis. International Journal of Molecular Sciences. 2017; 18(1):1. https://doi.org/10.3390/ijms18010001
Chicago/Turabian StyleWang, Haidong, Juan Wang, Yun Bai, Jinwei Li, Lixin Li, and Yanjun Dong. 2017. "CD11c+ CD8+ T Cells Reduce Renal Fibrosis Following Ureteric Obstruction by Inducing Fibroblast Apoptosis" International Journal of Molecular Sciences 18, no. 1: 1. https://doi.org/10.3390/ijms18010001