Toxicity of Pekinenin C from Euphorbia Pekinensis Radix on Rat Small Intestinal Crypt Epithelial Cell and Its Apoptotic Mechanism
Abstract
:1. Introduction
2. Results
2.1. Effects of Pekinenin C (PC) on Cell Viability Analysis
2.2. Effects of PC on Cell Cycle
2.3. Effects of PC on Cell Apoptosis
2.4. Effects of PC on Caspase-Dependent Mitochondria Pathway
2.5. Effects of PC on Death Receptor Pathway
3. Discussion
4. Experimental Section
4.1. Plant Materials and the Preparation of PC
4.2. Chemicals and Regents
4.3. Cell Culture
4.4. Cytotoxicity Assay
4.5. Cell Cycle Analysis
4.6. Cell Morphological Detection and Transmission Electron Microscopy (TEM)
4.7. Western Blot Analysis
4.8. Caspase-3, 8, 9 Activation Assay
4.9. RNA Isolation and Real Time Quantitative PCR
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Cao, Y.; Cheng, F.; Yao, W.; Bao, B.; Zhang, K.; Zhang, L.; Ding, A. Toxicity of Pekinenin C from Euphorbia Pekinensis Radix on Rat Small Intestinal Crypt Epithelial Cell and Its Apoptotic Mechanism. Int. J. Mol. Sci. 2016, 17, 850. https://doi.org/10.3390/ijms17060850
Cao Y, Cheng F, Yao W, Bao B, Zhang K, Zhang L, Ding A. Toxicity of Pekinenin C from Euphorbia Pekinensis Radix on Rat Small Intestinal Crypt Epithelial Cell and Its Apoptotic Mechanism. International Journal of Molecular Sciences. 2016; 17(6):850. https://doi.org/10.3390/ijms17060850
Chicago/Turabian StyleCao, Yudan, Fangfang Cheng, Weifeng Yao, Beihua Bao, Kaicheng Zhang, Li Zhang, and Anwei Ding. 2016. "Toxicity of Pekinenin C from Euphorbia Pekinensis Radix on Rat Small Intestinal Crypt Epithelial Cell and Its Apoptotic Mechanism" International Journal of Molecular Sciences 17, no. 6: 850. https://doi.org/10.3390/ijms17060850