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Int. J. Mol. Sci. 2016, 17(5), 740;

Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection

Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou 510380, Guangdong, China
These authors contributed equally to the work.
Authors to whom correspondence should be addressed.
Academic Editors: Jun Li and Helmut Segner
Received: 12 February 2016 / Revised: 10 April 2016 / Accepted: 10 May 2016 / Published: 19 May 2016
(This article belongs to the Special Issue Fish Molecular Biology)
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Nervous necrosis virus (NNV) is the causative agent of viral encephalopathy and retinopathy (VER), a neurological disease responsible for high mortality of fish species worldwide. Taking advantage of our established Chinese perch brain (CPB) cell line derived from brain tissues of Mandarin fish (Siniperca chuatsi), the susceptibility of CPB cell to Red-Spotted Grouper nervous necrosis virus (RGNNV) was evaluated. The results showed that RGNNV replicated well in CPB cells, resulting in cellular apoptosis. Moreover, the susceptibility of Mandarin fish to RGNNV was also evaluated. Abnormal swimming was observed in RGNNV-infected Mandarin fish. In addition, the cellular vacuolation and viral particles were also observed in brain tissues of RGNNV-infected Mandarin fish by Hematoxylin-eosin staining or electronic microscopy. The established RGNNV susceptible brain cell line from freshwater fish will pave a new way for the study of the pathogenicity and replication of NNV in the future. View Full-Text
Keywords: Mandarin fish; NNV; CPB; brain cell line; susceptibility Mandarin fish; NNV; CPB; brain cell line; susceptibility

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Tu, J.; Chen, W.; Fu, X.; Lin, Q.; Chang, O.; Zhao, L.; Lan, J.; Li, N.; Lin, L. Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection. Int. J. Mol. Sci. 2016, 17, 740.

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