Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46
Abstract
:1. Introduction
2. Results
2.1. miR-1894 Is a Novel Metastasis Suppressor MicroRNA for Mouse Breast Cancer
2.2. Mmu-miR-1894-3p Down-Regulated Trim46 by Binding to the Trim46 3′-Untranslated Region (UTR)
2.3. Trim46 Knockdown Inhibited Cell Proliferation and Metastasis in 4TO7 Mouse Breast Cancer Cells
2.4. Trim46 Was Involved in the Cell Proliferation and Migration of Human Breast Cancer Cells
2.5. Trim46 Knockdown by Mmu-miR-1894-3p or shRNA Reduced Its Expression on the Cell Surface
3. Discussion
4. Materials and Methods
4.1. Constructs
4.2. Cell Culture
4.3. qRT-PCR
4.4. Western Blot
4.5. Target Prediction
4.6. Dual-Luciferase Reporter Assays
4.7. In vivo Metastasis Assays
4.8. RTCA Assays
4.9. Immunofluorescence
4.10. Wound Healing Assay
4.11. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Zhang, L.; Li, X.; Dong, W.; Sun, C.; Guo, D.; Zhang, L. Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46. Int. J. Mol. Sci. 2016, 17, 609. https://doi.org/10.3390/ijms17040609
Zhang L, Li X, Dong W, Sun C, Guo D, Zhang L. Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46. International Journal of Molecular Sciences. 2016; 17(4):609. https://doi.org/10.3390/ijms17040609
Chicago/Turabian StyleZhang, Li, Xiaoying Li, Wei Dong, Caixian Sun, Deyu Guo, and Lianfeng Zhang. 2016. "Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46" International Journal of Molecular Sciences 17, no. 4: 609. https://doi.org/10.3390/ijms17040609