Characterization of Stripe Rust Resistance Genes in the Wheat Cultivar Chuanmai45
by
Ennian Yang 1,*, Guangrong Li 2, Liping Li 1, Zhenyu Zhang 3, Wuyun Yang 1, Yunliang Peng 3, Yongqing Zhu 4, Zujun Yang 2 and Garry M. Rosewarne 1,5,6
Ennian Yang 1,*, Guangrong Li 2, Liping Li 1, Zhenyu Zhang 3, Wuyun Yang 1, Yunliang Peng 3, Yongqing Zhu 4, Zujun Yang 2 and Garry M. Rosewarne 1,5,6
1
Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
2
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
3
Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
4
Institute of Agro Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
5
International Maize and Wheat Improvement Centre (CIMMYT), Apdo. Postal 6-6-41, Mexico 06600, D.F., Mexico
6
Department of Environment and Primary Industries, 110 Natimuk Rd, Horsham, Victoria 3401, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Int. J. Mol. Sci. 2016, 17(4), 601; https://doi.org/10.3390/ijms17040601
Received: 24 December 2015 / Revised: 14 April 2016 / Accepted: 15 April 2016 / Published: 21 April 2016
(This article belongs to the Special Issue Plant Innate Immunity)
The objective of this research was to characterize the high level of resistance to stripe that has been observed in the released wheat cultivar, Chuanmai45. A combination of classic genetic analysis, molecular and cytogenetic methods were used to characterize resistance in an F2 population derived from Chuanmai45 and the susceptible Chuanmai42. Inheritance of resistance was shown to be conferred by two genes in Chuanmai45. Fluorescence in situ hybridization (FISH) was used along with segregation studies to show that one gene was located on a 1RS.1BL translocation. Molecular markers were employed to show that the other locus was located on chromosome 4B. The defeated gene, Yr24/26, on chromosome 1BL was present in the susceptible parent and lines that recombined this gene with the 1RS.1BL translocation were identified. The germplasm, loci, and associated markers identified in this study will be useful for application in breeding programs utilizing marker-assisted selection.
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MDPI and ACS Style
Yang, E.; Li, G.; Li, L.; Zhang, Z.; Yang, W.; Peng, Y.; Zhu, Y.; Yang, Z.; Rosewarne, G.M. Characterization of Stripe Rust Resistance Genes in the Wheat Cultivar Chuanmai45. Int. J. Mol. Sci. 2016, 17, 601.
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