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Open AccessArticle

Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed

1
Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St., Albany, CA 94710, USA
2
Institute of Biological Chemistry, Washington State University, Pullman, Washington, DC 99164, USA
3
Department of Plant Biology and Crop Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Ute Roessner
Int. J. Mol. Sci. 2016, 17(4), 507; https://doi.org/10.3390/ijms17040507
Received: 4 March 2016 / Revised: 25 March 2016 / Accepted: 30 March 2016 / Published: 6 April 2016
(This article belongs to the Special Issue Metabolomics in the Plant Sciences)
Lesquerella is a potential industrial oilseed crop that makes hydroxy fatty acid (HFA). Unlike castor its seeds are not poisonous but accumulate lesquerolic acid mostly at the sn-1 and sn-3 positions of triacylglycerol (TAG), whereas castor contains ricinoleic acid (18:1OH) at all three positions. To investigate whether lesquerella can be engineered to accumulate HFAs in the sn-2 position, multiple transgenic lines were made that express castor lysophosphatidic acid acyltransferase 2 (RcLPAT2) in the seed. RcLPAT2 increased 18:1OH at the sn-2 position of TAGs from 2% to 14%–17%, which resulted in an increase of tri-HFA-TAGs from 5% to 13%–14%. Our result is the first example of using a LPAT to increase ricinoleic acid at the sn-2 position of seed TAG. This work provides insights to the mechanism of HFA-containing TAG assembly in lesquerella and directs future research to optimize this plant for HFA production. View Full-Text
Keywords: lesquerella; Physaria fendleri; lysophosphatidic acid acyltransferase; hydroxy fatty acid; seed triacylglycerol; metabolic engineering lesquerella; Physaria fendleri; lysophosphatidic acid acyltransferase; hydroxy fatty acid; seed triacylglycerol; metabolic engineering
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Chen, G.Q.; Van Erp, H.; Martin-Moreno, J.; Johnson, K.; Morales, E.; Browse, J.; Eastmond, P.J.; Lin, J.-T. Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed. Int. J. Mol. Sci. 2016, 17, 507.

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