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Int. J. Mol. Sci. 2016, 17(4), 382;

RTN3 Regulates the Expression Level of Chemokine Receptor CXCR4 and is Required for Migration of Primordial Germ Cells

State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China
Author to whom correspondence should be addressed.
Academic Editors: Kathleen Van Craenenbroeck and Constantinos Stathopoulos
Received: 6 January 2016 / Revised: 23 February 2016 / Accepted: 3 March 2016 / Published: 8 April 2016
(This article belongs to the Collection G Protein-Coupled Receptor Signaling and Regulation)
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CXCR4 is a crucial chemokine receptor that plays key roles in primordial germ cell (PGC) homing. To further characterize the CXCR4-mediated migration of PGCs, we screened CXCR4-interacting proteins using yeast two-hybrid screening. We identified reticulon3 (RTN3), a member of the reticulon family, and considered an apoptotic signal transducer, as able to interact directly with CXCR4. Furthermore, we discovered that the mRNA and protein expression levels of CXCR4 could be regulated by RTN3. We also found that RTN3 altered CXCR4 translocation and localization. Moreover, increasing the signaling of either CXCR4b or RTN3 produced similar PGC mislocalization phenotypes in zebrafish. These results suggested that RTN3 modulates PGC migration through interaction with, and regulation of, CXCR4. View Full-Text
Keywords: primordial germ cells; RTN3; CXCR4 primordial germ cells; RTN3; CXCR4

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Li, H.; Liang, R.; Lu, Y.; Wang, M.; Li, Z. RTN3 Regulates the Expression Level of Chemokine Receptor CXCR4 and is Required for Migration of Primordial Germ Cells. Int. J. Mol. Sci. 2016, 17, 382.

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