Next Article in Journal
Probing the Ion Binding Site in a DNA Holliday Junction Using Förster Resonance Energy Transfer (FRET)
Previous Article in Journal
Co-Expression and Co-Localization of Cartilage Glycoproteins CHI3L1 and Lubricin in Osteoarthritic Cartilage: Morphological, Immunohistochemical and Gene Expression Profiles
Open AccessArticle

Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
*
Author to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Int. J. Mol. Sci. 2016, 17(3), 357; https://doi.org/10.3390/ijms17030357
Received: 5 February 2016 / Revised: 28 February 2016 / Accepted: 4 March 2016 / Published: 10 March 2016
(This article belongs to the Section Biochemistry)
Background: Electroacupuncture (EA) tolerance is a gradual decline in EA antinociception because of its repeated or prolonged use. This study aims to explore the role of spinal glutamate transporters (GTs) in EA tolerance (EAT). Methods: Rats were treated with EA once per day for eight consecutive days, and their L4-5 spinal cords were collected at days 0, 2, 4, 6 and 8. The levels of three spinal GTs and their mRNAs were detected with Western blot and pPCR, respectively. Then, riluzole, a positive GT regulator, was administered intrathecally in order to observe its effect on EA analgesia after repeated EA. Results: The expression levels of the spinal GTs increased at days 2 and 4, and gradually decreased as the times of EA increased. At day 8, no difference was observed in the spinal GTs between the sham treatment and the EA treatment. Intrathecal administration of riluzole dose-dependently attenuated the decreased EA analgesia. Conclusion: These results indicated the participation of the spinal GTs in EAT. View Full-Text
Keywords: electroacupuncture tolerance; GLAST; GLT-1; EAAC1; riluzole; tail flick latency; rat electroacupuncture tolerance; GLAST; GLT-1; EAAC1; riluzole; tail flick latency; rat
Show Figures

Figure 1

MDPI and ACS Style

Cui, L.; Ding, Y.; Zeng, J.; Feng, Y.; Li, M.; Ding, M. Spinal Glutamate Transporters Are Involved in the Development of Electroacupuncture Tolerance. Int. J. Mol. Sci. 2016, 17, 357.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop