Next Article in Journal
ERα Mediates Estrogen-Induced Expression of the Breast Cancer Metastasis Suppressor Gene BRMS1
Next Article in Special Issue
Atrogin-1 Deficiency Leads to Myopathy and Heart Failure in Zebrafish
Previous Article in Journal
miR-16-5p Is a Stably-Expressed Housekeeping MicroRNA in Breast Cancer Tissues from Primary Tumors and from Metastatic Sites
Previous Article in Special Issue
Elucidating the Diversity of Aquatic Microdochium and Trichoderma Species and Their Activity against the Fish Pathogen Saprolegnia diclina
Article

Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection

1
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
2
Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China
3
Department of Zoology, Hazara University, Mansehra, Khyber Pakhtoonkhwa 21300, Pakistan
4
Central Laboratory for Environmental Quality Monitoring (CLEQM), National Water Research Center (NWRC), Cairo 13621, Egypt
5
Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD 21202, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to the work.
Academic Editor: Jun Li
Int. J. Mol. Sci. 2016, 17(2), 154; https://doi.org/10.3390/ijms17020154
Received: 5 January 2016 / Revised: 19 January 2016 / Accepted: 21 January 2016 / Published: 26 January 2016
(This article belongs to the Special Issue Fish Molecular Biology)
MicroRNAs (miRNAs) play important roles in mediating multiple biological processes in eukaryotes and are being increasingly studied to evaluate their roles associated with cellular changes following viral infection. Snakehead fish Vesiculovirus (SHVV) has caused mass mortality in snakehead fish during the past few years. To identify specific miRNAs involved in SHVV infection, we performed microRNA deep sequencing on a snakehead fish cell line (SSN-1) with or without SHVV infection. A total of 205 known miRNAs were identified when they were aligned with the known zebrafish miRNAs, and nine novel miRNAs were identified using MiRDeep2 software. Eighteen and 143 of the 205 known miRNAs were differentially expressed at three and 24 h post-infection (poi), respectively. From the differentially-expressed miRNAs, five were randomly selected to validate their expression profiles using quantitative reverse transcription polymerase chain reaction (qRT-PCR), and their expression profiles were consistent with the microRNA sequencing results. In addition, the target gene prediction of the SHVV genome was performed for the differentially-expressed host miRNAs, and a total of 10 and 58 differentially-expressed miRNAs were predicted to bind to the SHVV genome at three and 24 h poi, respectively. The effects of three selected miRNAs (miR-130-5p, miR-214 and miR-216b) on SHVV multiplication were evaluated using their mimics and inhibitors via qRT-PCR and Western blotting. The results showed that all three miRNAs were able to inhibit the multiplication of SHVV; whereas the mechanisms underlying the SHVV multiplication inhibited by the specific miRNAs need to be further characterized in the future. View Full-Text
Keywords: snakehead fish Vesiculovirus; SSN-1 cell; miRNA; deep sequencing snakehead fish Vesiculovirus; SSN-1 cell; miRNA; deep sequencing
Show Figures

Graphical abstract

MDPI and ACS Style

Liu, X.; Tu, J.; Yuan, J.; Liu, X.; Zhao, L.; Dawar, F.U.; Khattak, M.N.K.; Hegazy, A.M.; Chen, N.; Vakharia, V.N.; Lin, L. Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection. Int. J. Mol. Sci. 2016, 17, 154. https://doi.org/10.3390/ijms17020154

AMA Style

Liu X, Tu J, Yuan J, Liu X, Zhao L, Dawar FU, Khattak MNK, Hegazy AM, Chen N, Vakharia VN, Lin L. Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection. International Journal of Molecular Sciences. 2016; 17(2):154. https://doi.org/10.3390/ijms17020154

Chicago/Turabian Style

Liu, Xiaodan; Tu, Jiagang; Yuan, Junfa; Liu, Xueqin; Zhao, Lijuan; Dawar, Farman U.; Khattak, Muhammad N.K.; Hegazy, Abeer M.; Chen, Nan; Vakharia, Vikram N.; Lin, Li. 2016. "Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection" Int. J. Mol. Sci. 17, no. 2: 154. https://doi.org/10.3390/ijms17020154

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop