Next Article in Journal
Discovery of Anti-Hypertensive Oligopeptides from Adlay Based on In Silico Proteolysis and Virtual Screening
Next Article in Special Issue
Cellular Reprogramming Using Protein and Cell-Penetrating Peptides
Previous Article in Journal
Data Analysis in Chemistry and Bio-Medical Sciences
Previous Article in Special Issue
The Telomerase-Derived Anticancer Peptide Vaccine GV1001 as an Extracellular Heat Shock Protein-Mediated Cell-Penetrating Peptide
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessCommunication
Int. J. Mol. Sci. 2016, 17(12), 2104;

Cre Fused with RVG Peptide Mediates Targeted Genome Editing in Mouse Brain Cells In Vivo

State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China
College of Life Science, Liaoning University, Shenyang 110036, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editors: Jagdish Singh and Buddhadev Layek
Received: 19 October 2016 / Revised: 2 December 2016 / Accepted: 8 December 2016 / Published: 14 December 2016
(This article belongs to the Special Issue Cell-Penetrating Peptides 2016)
Full-Text   |   PDF [1502 KB, uploaded 14 December 2016]   |  


Cell penetrating peptides (CPPs) are short peptides that can pass through cell membranes. CPPs can facilitate the cellular entry of proteins, macromolecules, nanoparticles and drugs. RVG peptide (RVG hereinafter) is a 29-amino-acid CPP derived from a rabies virus glycoprotein that can cross the blood-brain barrier (BBB) and enter brain cells. However, whether RVG can be used for genome editing in the brain has not been reported. In this work, we combined RVG with Cre recombinase for bacterial expression. The purified RVG-Cre protein cut plasmids in vitro and traversed cell membranes in cultured Neuro2a cells. By tail vein-injecting RVG-Cre into Cre reporter mouse lines mTmG and Rosa26lacZ, we demonstrated that RVG-Cre could target brain cells and achieve targeted somatic genome editing in adult mice. This direct delivery of the gene-editing enzyme protein into mouse brains with RVG is much safer than plasmid- or viral-based methods, holding promise for further applications in the treatment of various brain diseases. View Full-Text
Keywords: genome editing; RVG peptide; Cre; blood brain barrier genome editing; RVG peptide; Cre; blood brain barrier

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Zou, Z.; Sun, Z.; Li, P.; Feng, T.; Wu, S. Cre Fused with RVG Peptide Mediates Targeted Genome Editing in Mouse Brain Cells In Vivo. Int. J. Mol. Sci. 2016, 17, 2104.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top