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Int. J. Mol. Sci. 2016, 17(10), 1612;

Elevated Expression of Calpain-4 Predicts Poor Prognosis in Patients with Gastric Cancer after Gastrectomy

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Institute of Biomedical Science, Fudan University, Shanghai 200032, China
Authors to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 26 July 2016 / Revised: 14 September 2016 / Accepted: 19 September 2016 / Published: 27 September 2016
(This article belongs to the Collection Advances in Molecular Oncology)
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Calpain-4 belongs to the calpain family of calcium-dependent cysteine proteases, and functions as a small regulatory subunit of the calpains. Recent evidence indicates that calpain-4 plays critical roles in tumor migration and invasion. However, the roles of calpain-4 in gastric tumorigenesis remain poorly understood. Herein, we examined calpain-4 expression by immunohistochemical staining on tissue microarrays containing tumor samples of 174 gastric cancer patients between 2004 and 2008 at a single center. The Kaplan-Meier method was used to compare survival curves, and expression levels were correlated to clinicopathological factors and overall survival. Our data demonstrated that calpain-4 was generally increased in gastric cancer cell lines and primary tumor tissues. High expression of calpain-4 was positively associated with vessel invasion, lymph node metastasis, and advanced TNM (Tumor Node Metastasis) stage. Multivariate analysis identified calpain-4 as an independent prognostic factor for poor prognosis. A predictive nomogram integrating calpain-4 expression with other independent prognosticators was constructed, which generated a better prognostic value for overall survival of gastric cancer patients than a TNM staging system. In conclusion, calpain-4 could be regarded as a potential prognosis indicator for clinical outcomes in gastric cancer. View Full-Text
Keywords: gastric cancer; calpain-4; prognosis; biomarker; overall survival; nomogram gastric cancer; calpain-4; prognosis; biomarker; overall survival; nomogram

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Peng, P.; Min, L.; Song, S.; Zhao, J.; Li, L.; Yang, C.; Shao, M.; Zhang, M.; Wu, H.; Zhang, J.; Li, C.; Wang, X.; Wang, H.; Qin, J.; Ruan, Y.; Gu, J. Elevated Expression of Calpain-4 Predicts Poor Prognosis in Patients with Gastric Cancer after Gastrectomy. Int. J. Mol. Sci. 2016, 17, 1612.

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