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Int. J. Mol. Sci. 2016, 17(10), 1568;

Identification of Resistance to Wet Bubble Disease and Genetic Diversity in Wild and Cultivated Strains of Agaricus bisporus

Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China
The Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350014, China
Department of Crop and Soil Sciences, Washington State University, Pullman, Washington, DC 99163, USA
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 10 July 2016 / Revised: 5 September 2016 / Accepted: 9 September 2016 / Published: 22 September 2016
(This article belongs to the Section Molecular Plant Sciences)
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Outbreaks of wet bubble disease (WBD) caused by Mycogone perniciosa are increasing across the world and seriously affecting the yield of Agaricus bisporus. However, highly WBD-resistant strains are rare. Here, we tested 28 A. bisporus strains for WBD resistance by inoculating M. perniciosa spore suspension on casing soil, and assessed genetic diversity of these strains using 17 new simple sequence repeat (SSR) markers developed in this study. We found that 10 wild strains originating from the Tibetan Plateau in China were highly WBD-resistant strains, and 13 cultivated strains from six countries were highly susceptible strains. A total of 88 alleles were detected in these 28 strains, and the observed number of alleles per locus ranged from 2 to 8. Cluster and genetic structure analysis results revealed the wild resources from China have a relatively high level of genetic diversity and occur at low level of gene flow and introgression with cultivated strains. Moreover, the wild strains from China potentially have the consensus ancestral genotypes different from the cultivated strains and evolved independently. Therefore, the highly WBD-resistant wild strains from China and newly developed SSR markers could be used as novel sources for WBD-resistant breeding and quantitative trait locus (QTL) mapping of WBD-resistant gene of A. bisporus. View Full-Text
Keywords: Agaricus bisporus; Mycogone perniciosa; highly resistant; SSR; population structure Agaricus bisporus; Mycogone perniciosa; highly resistant; SSR; population structure

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Fu, Y.; Wang, X.; Li, D.; Liu, Y.; Song, B.; Zhang, C.; Wang, Q.; Chen, M.; Zhang, Z.; Li, Y. Identification of Resistance to Wet Bubble Disease and Genetic Diversity in Wild and Cultivated Strains of Agaricus bisporus. Int. J. Mol. Sci. 2016, 17, 1568.

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