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Influence of Bxpel1 Gene Silencing by dsRNA Interference on the Development and Pathogenicity of the Pine Wood Nematode, Bursaphelenchus xylophilus

1
Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing 210037, China
2
Poyang Lake Eco-economy Research Center, Jiujiang University, Jiujiang 332005, China
*
Author to whom correspondence should be addressed.
Academic Editor: Michael Ladomery
Int. J. Mol. Sci. 2016, 17(1), 125; https://doi.org/10.3390/ijms17010125
Received: 21 October 2015 / Revised: 12 January 2016 / Accepted: 14 January 2016 / Published: 19 January 2016
(This article belongs to the Section Biochemistry)
As the causal agent of pine wilt disease (PWD), the pine wood nematode (PWN), Bursaphelenchus xylophilus, causes huge economic losses by devastating pine forests worldwide. The pectate lyase gene is essential for successful invasion of their host plants by plant-parasitic nematodes. To demonstrate the role of pectate lyase gene in the PWD process, RNA interference (RNAi) is used to analyze the function of the pectate lyase 1 gene in B. xylophilus (Bxpel1). The efficiency of RNAi was detected by real-time PCR. The result demonstrated that the quantity of B. xylophilus propagated with control solution treatment was 62 times greater than that soaking in double-stranded RNA (dsRNA) after B. xylophilus inoculation in Botrytis cinerea for the first generation (F1). The number of B. xylophilus soaking in control solution was doubled compared to that soaking in Bxpel1 dsRNA four days after inoculation in Pinus thunbergii. The quantity of B. xylophilus was reduced significantly (p < 0.001) after treatment with dsRNAi compared with that using a control solution treatment. Bxpel1 dsRNAi reduced the migration speed and reproduction of B. xylophilus in pine trees. The pathogenicity to P. thunbergii seedling of B. xylophilus was weaker after soaking in dsRNA solution compared with that after soaking in the control solution. Our results suggest that Bxpel1 gene is a significant pathogenic factor in the PWD process and this basic information may facilitate a better understanding of the molecular mechanism of PWD. View Full-Text
Keywords: Bursaphelenchus xylophilus; pathogenicity; pectate lyase; RNA interference (RNAi) Bursaphelenchus xylophilus; pathogenicity; pectate lyase; RNA interference (RNAi)
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MDPI and ACS Style

Qiu, X.-W.; Wu, X.-Q.; Huang, L.; Ye, J.-R. Influence of Bxpel1 Gene Silencing by dsRNA Interference on the Development and Pathogenicity of the Pine Wood Nematode, Bursaphelenchus xylophilus. Int. J. Mol. Sci. 2016, 17, 125. https://doi.org/10.3390/ijms17010125

AMA Style

Qiu X-W, Wu X-Q, Huang L, Ye J-R. Influence of Bxpel1 Gene Silencing by dsRNA Interference on the Development and Pathogenicity of the Pine Wood Nematode, Bursaphelenchus xylophilus. International Journal of Molecular Sciences. 2016; 17(1):125. https://doi.org/10.3390/ijms17010125

Chicago/Turabian Style

Qiu, Xiu-Wen; Wu, Xiao-Qin; Huang, Lin; Ye, Jian-Ren. 2016. "Influence of Bxpel1 Gene Silencing by dsRNA Interference on the Development and Pathogenicity of the Pine Wood Nematode, Bursaphelenchus xylophilus" Int. J. Mol. Sci. 17, no. 1: 125. https://doi.org/10.3390/ijms17010125

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