Next Article in Journal
Cloning and Transcriptional Activity of the Mouse Omi/HtrA2 Gene Promoter
Next Article in Special Issue
N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability
Previous Article in Journal
Direct Analysis in Real Time (DART) of an Organothiophosphate at Ultrahigh Resolution by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and Tandem Mass Spectrometry
Previous Article in Special Issue
Post-Translational Modification and Secretion of Azelaic Acid Induced 1 (AZI1), a Hybrid Proline-Rich Protein from Arabidopsis
Open AccessArticle

Ultra-Fast Glyco-Coating of Non-Biological Surfaces

1
Centre for Kode Technology Innovation, Faculty of Design and Creative Technologies, Auckland University of Technology, Private Bag 92006, Auckland 1142, New Zealand
2
Laboratory of Carbohydrate Chemistry, Shemyakin Institute of Bioorganic Chemistry, Moscow 117997, Russia
*
Authors to whom correspondence should be addressed.
Academic Editor: Patricia Berninsone
Int. J. Mol. Sci. 2016, 17(1), 118; https://doi.org/10.3390/ijms17010118
Received: 30 November 2015 / Revised: 23 December 2015 / Accepted: 31 December 2015 / Published: 16 January 2016
(This article belongs to the Special Issue Glycosylation and Glycoproteins)
The ability to glycosylate surfaces has medical and diagnostic applications, but there is no technology currently recognized as being able to coat any surface without the need for prior chemical modification of the surface. Recently, a family of constructs called function-spacer-lipids (FSL) has been used to glycosylate cells. Because it is known that lipid-based material can adsorb onto surfaces, we explored the potential and performance of cell-labelling FSL constructs to “glycosylate” non-biological surfaces. Using blood group A antigen as an indicator, the performance of a several variations of FSL constructs to modify a large variety of non-biological surfaces was evaluated. It was found the FSL constructs when optimised could in a few seconds glycosylate almost any non-biological surface including metals, glass, plastics, rubbers and other polymers. Although the FSL glycan coating was non-covalent, and therefore temporary, it was sufficiently robust with appropriate selection of spacer and surface that it could capture anti-glycan antibodies, immobilize cells (via antibody), and withstand incubation in serum and extensive buffer washing, making it suitable for diagnostic and research applications. View Full-Text
Keywords: function-spacer-lipid; blood group A; nanofibres; surface-coating; glyco-coating; glyco-landscape; shear stress function-spacer-lipid; blood group A; nanofibres; surface-coating; glyco-coating; glyco-landscape; shear stress
Show Figures

Graphical abstract

MDPI and ACS Style

Williams, E.; Barr, K.; Korchagina, E.; Tuzikov, A.; Henry, S.; Bovin, N. Ultra-Fast Glyco-Coating of Non-Biological Surfaces. Int. J. Mol. Sci. 2016, 17, 118.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop