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Open AccessArticle

The Role of PTP1B O-GlcNAcylation in Hepatic Insulin Resistance

Department of Endocrinology, Affiliated Hospital of Nantong University, Nantong 226001, China
Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong 226001, China
Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Patricia Berninsone
Int. J. Mol. Sci. 2015, 16(9), 22856-22869;
Received: 11 May 2015 / Revised: 1 September 2015 / Accepted: 16 September 2015 / Published: 22 September 2015
(This article belongs to the Special Issue Glycosylation and Glycoproteins)
Protein tyrosine phosphatase 1B (PTP1B), which can directly dephosphorylate both the insulin receptor and insulin receptor substrate 1 (IRS-1), thereby terminating insulin signaling, reportedly plays an important role in insulin resistance. Accumulating evidence has demonstrated that O-GlcNAc modification regulates functions of several important components of insulin signal pathway. In this study, we identified that PTP1B is modified by O-GlcNAcylation at three O-GlcNAc sites (Ser104, Ser201, and Ser386). Palmitate acid (PA) impaired the insulin signaling, indicated by decreased phosphorylation of both serine/threonine-protein kinase B (Akt) and glycogen synthase kinase 3 beta (GSK3β) following insulin administration, and upregulated PTP1B O-GlcNAcylation in HepG2 cells. Compared with the wild-type, intervention PTP1B O-GlcNAcylation by site-directed gene mutation inhibited PTP1B phosphatase activity, resulted in a higher level of phosphorylated Akt and GSK3β, recovered insulin sensitivity, and improved lipid deposition in HepG2 cells. Taken together, our research showed that O-GlcNAcylation of PTP1B can influence insulin signal transduction by modulating its own phosphatase activity, which participates in the process of hepatic insulin resistance. View Full-Text
Keywords: insulin resistance; PTP1B; O-GlcNAc insulin resistance; PTP1B; O-GlcNAc
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MDPI and ACS Style

Zhao, Y.; Tang, Z.; Shen, A.; Tao, T.; Wan, C.; Zhu, X.; Huang, J.; Zhang, W.; Xia, N.; Wang, S.; Cui, S.; Zhang, D. The Role of PTP1B O-GlcNAcylation in Hepatic Insulin Resistance. Int. J. Mol. Sci. 2015, 16, 22856-22869.

AMA Style

Zhao Y, Tang Z, Shen A, Tao T, Wan C, Zhu X, Huang J, Zhang W, Xia N, Wang S, Cui S, Zhang D. The Role of PTP1B O-GlcNAcylation in Hepatic Insulin Resistance. International Journal of Molecular Sciences. 2015; 16(9):22856-22869.

Chicago/Turabian Style

Zhao, Yun; Tang, Zhuqi; Shen, Aiguo; Tao, Tao; Wan, Chunhua; Zhu, Xiaohui; Huang, Jieru; Zhang, Wanlu; Xia, Nana; Wang, Suxin; Cui, Shiwei; Zhang, Dongmei. 2015. "The Role of PTP1B O-GlcNAcylation in Hepatic Insulin Resistance" Int. J. Mol. Sci. 16, no. 9: 22856-22869.

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