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Open AccessArticle

NOS1AP O-GlcNAc Modification Involved in Neuron Apoptosis Induced by Excitotoxicity

by Liang Zhu 1,2,†, Tao Tao 1,2,†, Dongmei Zhang 1,2, Xiaojuan Liu 1,2, Kaifu Ke 1,* and Aiguo Shen 1,2,3,*
1
Department of Neurology, Affiliated Hospital of Nantong University, the Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong 226000, China
2
The Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong 226000, China
3
Department of Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226000, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Joe Tiralongo and Patricia Berninsone
Int. J. Mol. Sci. 2015, 16(7), 16560-16575; https://doi.org/10.3390/ijms160716560
Received: 11 May 2015 / Revised: 5 June 2015 / Accepted: 16 June 2015 / Published: 21 July 2015
(This article belongs to the Special Issue Glycosylation and Glycoproteins)
O-Linked N-acetylglucosamine, or O-GlcNAc, is a dynamic post-translational modification that cycles on and off serine and threonine residues of nucleocytoplasmic and mitochondrial proteins. In addition to cancer and inflammation diseases, O-GlcNAc modification appears to play a critical role during cell apoptosis and stress response, although the precise mechanisms are still not very clear. Here we found that nitric oxide synthase adaptor (NOS1AP), which plays an important part in glutamate-induced neuronal apoptosis, carries the modification of O-GlcNAc. Mass spectrometry analysis identified Ser47, Ser183, Ser204, Ser269, Ser271 as O-GlcNAc sites. Higher O-GlcNAc of NOS1AP was detected during glutamate-induced neuronal apoptosis. Furthermore, with O-GlcNAc sites of NOS1AP mutated, the interaction of NOS1AP and neuronal nitric oxide syntheses (nNOS) decreases. Finally, during glutamate-induced neuronal apoptosis, decreasing the O-GlcNAc modification of NOS1AP results in more severe neuronal apoptosis. All these results suggest that O-GlcNAc modification of NOS1AP exerts protective effects during glutamate-induced neuronal apoptosis. View Full-Text
Keywords: O-GlcNAc modification; NOS1AP; excitotoxicity O-GlcNAc modification; NOS1AP; excitotoxicity
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MDPI and ACS Style

Zhu, L.; Tao, T.; Zhang, D.; Liu, X.; Ke, K.; Shen, A. NOS1AP O-GlcNAc Modification Involved in Neuron Apoptosis Induced by Excitotoxicity. Int. J. Mol. Sci. 2015, 16, 16560-16575.

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