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Int. J. Mol. Sci. 2015, 16(9), 21454-21485;

The Proteome Profiles of the Cerebellum of Juvenile, Adult and Aged Rats—An Ontogenetic Study

Department of Anatomy, Gertrudenstr. 9, 18055 Rostock, Germany
Proteome Center Rostock, Schillingallee 69, 18055 Rostock, Germany
Department of Pathology, Josef-Schneider-Str. 2, 97080 Würzburg, Germany
Author to whom correspondence should be addressed.
Academic Editor: Lee A. Bulla
Received: 12 June 2015 / Revised: 4 August 2015 / Accepted: 25 August 2015 / Published: 7 September 2015
(This article belongs to the Collection Advances in Proteomic Research)
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In this study, we searched for proteins that change their expression in the cerebellum (Ce) of rats during ontogenesis. This study focuses on the question of whether specific proteins exist which are differentially expressed with regard to postnatal stages of development. A better characterization of the microenvironment and its development may result from these study findings. A differential two-dimensional polyacrylamide gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of the samples revealed that the number of proteins of the functional classes differed depending on the developmental stages. Especially members of the functional classes of biosynthesis, regulatory proteins, chaperones and structural proteins show the highest differential expression within the analyzed stages of development. Therefore, members of these functional protein groups seem to be involved in the development and differentiation of the Ce within the analyzed development stages. In this study, changes in the expression of proteins in the Ce at different postnatal developmental stages (postnatal days (P) 7, 90, and 637) could be observed. At the same time, an identification of proteins which are involved in cell migration and differentiation was possible. Especially proteins involved in processes of the biosynthesis and regulation, the dynamic organization of the cytoskeleton as well as chaperones showed a high amount of differentially expressed proteins between the analyzed dates. View Full-Text
Keywords: proteomics; rat; brain; cerebellum; development proteomics; rat; brain; cerebellum; development

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Wille, M.; Schümann, A.; Wree, A.; Kreutzer, M.; Glocker, M.O.; Mutzbauer, G.; Schmitt, O. The Proteome Profiles of the Cerebellum of Juvenile, Adult and Aged Rats—An Ontogenetic Study. Int. J. Mol. Sci. 2015, 16, 21454-21485.

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