Next Article in Journal
Detecting Antigen-Specific T Cell Responses: From Bulk Populations to Single Cells
Next Article in Special Issue
Overnutrition Determines LPS Regulation of Mycotoxin Induced Neurotoxicity in Neurodegenerative Diseases
Previous Article in Journal
From Genome to Structure and Back Again: A Family Portrait of the Transcarbamylases
Previous Article in Special Issue
Combination of Aβ Secretion and Oxidative Stress in an Alzheimer-Like Cell Line Leads to the Over-Expression of the Nucleotide Excision Repair Proteins DDB2 and XPC
Open AccessArticle

Major Alterations of Phosphatidylcholine and Lysophosphotidylcholine Lipids in the Substantia Nigra Using an Early Stage Model of Parkinson’s Disease

1
Carleton University Department of Neuroscience, 1125 Colonel By Drive, Life Sciences Research Building, Ottawa, ON K1S 5B6, Canada
2
Carleton University Department of Chemistry and Institute of Biochemistry, 1125 Colonel By Drive, Steacie Building, Ottawa, ON K1S 5B6, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: G. Jean Harry
Int. J. Mol. Sci. 2015, 16(8), 18865-18877; https://doi.org/10.3390/ijms160818865
Received: 18 June 2015 / Revised: 6 August 2015 / Accepted: 6 August 2015 / Published: 12 August 2015
(This article belongs to the Special Issue Molecular Research in Neurotoxicology)
Parkinson’s disease (PD) is a progressive neurodegenerative disease affecting the nigrostriatal pathway, where patients do not manifest motor symptoms until >50% of neurons are lost. Thus, it is of great importance to determine early neuronal changes that may contribute to disease progression. Recent attention has focused on lipids and their role in pro- and anti-apoptotic processes. However, information regarding the lipid alterations in animal models of PD is lacking. In this study, we utilized high performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) and novel HPLC solvent methodology to profile phosphatidylcholines and sphingolipids within the substantia nigra. The ipsilateral substantia nigra pars compacta was collected from rats 21 days after an infusion of 6-hydroxydopamine (6-OHDA), or vehicle into the anterior dorsal striatum. We identified 115 lipid species from their mass/charge ratio using the LMAPS Lipid MS Predict Database. Of these, 19 lipid species (from phosphatidylcholine and lysophosphotidylcholine lipid classes) were significantly altered by 6-OHDA, with most being down-regulated. The two lipid species that were up-regulated were LPC (16:0) and LPC (18:1), which are important for neuroinflammatory signalling. These findings provide a first step in the characterization of lipid changes in early stages of PD-like pathology and could provide novel targets for early interventions in PD. View Full-Text
Keywords: lipidomic profile; Parkinson’s disease; early stage model; 6-hydroxydopamine; HPLC-ESI-MS/MS lipidomic profile; Parkinson’s disease; early stage model; 6-hydroxydopamine; HPLC-ESI-MS/MS
Show Figures

Figure 1

MDPI and ACS Style

Farmer, K.; Smith, C.A.; Hayley, S.; Smith, J. Major Alterations of Phosphatidylcholine and Lysophosphotidylcholine Lipids in the Substantia Nigra Using an Early Stage Model of Parkinson’s Disease. Int. J. Mol. Sci. 2015, 16, 18865-18877.

AMA Style

Farmer K, Smith CA, Hayley S, Smith J. Major Alterations of Phosphatidylcholine and Lysophosphotidylcholine Lipids in the Substantia Nigra Using an Early Stage Model of Parkinson’s Disease. International Journal of Molecular Sciences. 2015; 16(8):18865-18877.

Chicago/Turabian Style

Farmer, Kyle; Smith, Catherine A.; Hayley, Shawn; Smith, Jeffrey. 2015. "Major Alterations of Phosphatidylcholine and Lysophosphotidylcholine Lipids in the Substantia Nigra Using an Early Stage Model of Parkinson’s Disease" Int. J. Mol. Sci. 16, no. 8: 18865-18877.

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop