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Int. J. Mol. Sci. 2015, 16(8), 18396-18411;

Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS

Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda Fuentenueva s/n, 18071 Granada, Spain
Research and Development Functional Food Centre, Health Science Technological Park, Avda Conocimiento s/n, 18016 Granada, Spain
Instituto de Biología Molecular y Celular (IBMC), Miguel Hernández University, Elche, 03202 Alicante, Spain
Pharmacokinetics and Pharmaceutical Technology Area, Engineering Department, Universidad Miguel Hernández, San Juan de Alicante, 03550 Alicante, Spain
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
These authors share co-senior authorship.
CIBER (CB12/03/30038, Fisiopatología de la Obesidad y la Nutrición, CIBERobn, Instituto de Salud Carlos III).
Academic Editors: Paula Andrade and Patrícia Valentão
Received: 6 July 2015 / Revised: 30 July 2015 / Accepted: 30 July 2015 / Published: 7 August 2015
(This article belongs to the Special Issue Phenolics and Polyphenolics 2015)
PDF [778 KB, uploaded 7 August 2015]


Previous findings on the capacity of Hibiscus sabdariffa (HS) polyphenols to ameliorate metabolic disturbances justify the necessity of studies oriented to find the potential metabolites responsible for such an effect. The present study examined the intestinal epithelial membrane permeability of polyphenols present in a phenolic-enriched Hibiscus sabdariffa extract (PEHS), free and encapsulated, using the Caco-2 cell line. Additionally, selected polyphenols (quercetin, quercetin-3-glucoside, quercetin-3-glucuronide, and N-feruloyltyramine) were also studied in the same absorption model. The powerful analytical platform used ultra-high-performance liquid chromatography coupled with ultra-high-resolution quadrupole time-of-flight mass spectrometry (UHPLC-ESI-UHR-Qq-TOF-MS), and enabled the characterization of seven new compounds in PEHS. In the permeation study, only a few compounds were able to cross the cell monolayer and the permeability was lower when the extract was in an encapsulated form. Pure compounds showed a moderate absorption in all cases. Nevertheless, these preliminary results may need further research to understand the complete absorption mechanism of Hibiscus polyphenols. View Full-Text
Keywords: Hibiscus sabdariffa; phenolic compounds; flavonoids; Caco-2 cells; UHPLC-ESI-UHR-Qq-TOF-MS Hibiscus sabdariffa; phenolic compounds; flavonoids; Caco-2 cells; UHPLC-ESI-UHR-Qq-TOF-MS

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Borrás-Linares, I.; Herranz-López, M.; Barrajón-Catalán, E.; Arráez-Román, D.; Gonzálezlvarez, I.; Bermejo, M.; Gutiérrez, A.F.; Micol, V.; Segura-Carretero, A. Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS. Int. J. Mol. Sci. 2015, 16, 18396-18411.

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