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Open AccessArticle

Identification of New Potential Interaction Partners for Human Cytoplasmic Copper Chaperone Atox1: Roles in Gene Regulation?

1
Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala 751 23, Sweden
2
Department of Chemistry, Umeå University, Umeå 901 87, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Masatoshi Maki
Int. J. Mol. Sci. 2015, 16(8), 16728-16739; https://doi.org/10.3390/ijms160816728
Received: 4 June 2015 / Revised: 30 June 2015 / Accepted: 20 July 2015 / Published: 23 July 2015
(This article belongs to the Special Issue Metalloproteins)
The human copper (Cu) chaperone Atox1 delivers Cu to P1B type ATPases in the Golgi network, for incorporation into essential Cu-dependent enzymes. Atox1 homologs are found in most organisms; it is a 68-residue ferredoxin-fold protein that binds Cu in a conserved surface-exposed Cys-X-X-Cys (CXXC) motif. In addition to its well-documented cytoplasmic chaperone function, in 2008 Atox1 was suggested to have functionality in the nucleus. To identify new interactions partners of Atox1, we performed a yeast two-hybrid screen with a large human placenta library of cDNA fragments using Atox1 as bait. Among 98 million fragments investigated, 25 proteins were found to be confident interaction partners. Nine of these were uncharacterized proteins, and the remaining 16 proteins were analyzed by bioinformatics with respect to cell localization, tissue distribution, function, sequence motifs, three-dimensional structures and interaction networks. Several of the hits were eukaryotic-specific proteins interacting with DNA or RNA implying that Atox1 may act as a modulator of gene regulation. Notably, because many of the identified proteins contain CXXC motifs, similarly to the Cu transport reactions, interactions between these and Atox1 may be mediated by Cu. View Full-Text
Keywords: copper chaperone; Atox1; transcription factor; two-hybrid screen; bioinformatics copper chaperone; Atox1; transcription factor; two-hybrid screen; bioinformatics
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Öhrvik, H.; Wittung-Stafshede, P. Identification of New Potential Interaction Partners for Human Cytoplasmic Copper Chaperone Atox1: Roles in Gene Regulation? Int. J. Mol. Sci. 2015, 16, 16728-16739.

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