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Int. J. Mol. Sci. 2015, 16(7), 15172-15187;

Transcriptome Sequencing of Lima Bean (Phaseolus lunatus) to Identify Putative Positive Selection in Phaseolus and Legumes

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing100193, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 20 March 2015 / Revised: 5 June 2015 / Accepted: 11 June 2015 / Published: 3 July 2015
(This article belongs to the Special Issue Plant Molecular Biology)
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The identification of genes under positive selection is a central goal of evolutionary biology. Many legume species, including Phaseolus vulgaris (common bean) and Phaseolus lunatus (lima bean), have important ecological and economic value. In this study, we sequenced and assembled the transcriptome of one Phaseolus species, lima bean. A comparison with the genomes of six other legume species, including the common bean, Medicago, lotus, soybean, chickpea, and pigeonpea, revealed 15 and 4 orthologous groups with signatures of positive selection among the two Phaseolus species and among the seven legume species, respectively. Characterization of these positively selected genes using Non redundant (nr) annotation, gene ontology (GO) classification, GO term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that these genes are mostly involved in thylakoids, photosynthesis and metabolism. This study identified genes that may be related to the divergence of the Phaseolus and legume species. These detected genes are particularly good candidates for subsequent functional studies. View Full-Text
Keywords: legume; lima bean; transcriptome; positive selection legume; lima bean; transcriptome; positive selection

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Li, F.; Cao, D.; Liu, Y.; Yang, T.; Wang, G. Transcriptome Sequencing of Lima Bean (Phaseolus lunatus) to Identify Putative Positive Selection in Phaseolus and Legumes. Int. J. Mol. Sci. 2015, 16, 15172-15187.

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