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Open AccessArticle

Do Matrix Metalloproteases and Tissue Inhibitors of Metalloproteases in Tenocytes of the Rotator Cuff Differ with Varying Donor Characteristics?

1
Julius Wolff Institute, Center for Musculoskeletal Surgery, Charité-Universitaetsmedizin Berlin, 13353 Berlin, Germany
2
Berlin-Brandenburg Center for Regenerative Therapies, Charité-Universitaetsmedizin Berlin, 13353 Berlin, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Christo Z. Christov
Int. J. Mol. Sci. 2015, 16(6), 13141-13157; https://doi.org/10.3390/ijms160613141
Received: 9 March 2015 / Accepted: 28 May 2015 / Published: 9 June 2015
(This article belongs to the Collection Proteins and Protein-Ligand Interactions)
An imbalance between matrix metalloproteases (MMPs) and the tissue inhibitors of metalloproteases (TIMPs) may have a negative impact on the healing of rotator cuff tears. The aim of the project was to assess a possible relationship between clinical and radiographic characteristics of patients such as the age, sex, as well as the degenerative status of the tendon and the MMPs and TIMPs in their tenocyte-like cells (TLCs). TLCs were isolated from ruptured supraspinatus tendons and quantitative Real-Time PCR and ELISA was performed to analyze the expression and secretion of MMPs and TIMPs. In the present study, MMPs, mostly gelatinases and collagenases such as MMP-2, -9 and -13 showed an increased expression and protein secretion in TLCs of donors with higher age or degenerative status of the tendon. Furthermore, the expression and secretion of TIMP-1, -2 and -3 was enhanced with age, muscle fatty infiltration and tear size. The interaction between MMPs and TIMPs is a complex process, since TIMPs are not only inhibitors, but also activators of MMPs. This study shows that MMPs and TIMPs might play an important role in degenerative tendon pathologies. View Full-Text
Keywords: tenocytes; rotator cuff; age; degeneration; MMPs; TIMPs tenocytes; rotator cuff; age; degeneration; MMPs; TIMPs
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Klatte-Schulz, F.; Aleyt, T.; Pauly, S.; Geißler, S.; Gerhardt, C.; Scheibel, M.; Wildemann, B. Do Matrix Metalloproteases and Tissue Inhibitors of Metalloproteases in Tenocytes of the Rotator Cuff Differ with Varying Donor Characteristics? Int. J. Mol. Sci. 2015, 16, 13141-13157.

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