Next Article in Journal
Consumption of Bilberries Controls Gingival Inflammation
Next Article in Special Issue
Hydrogen Production by the Thermophilic Bacterium Thermotoga neapolitana
Previous Article in Journal
Frequent Epigenetic Suppression of Tumor Suppressor Gene Glutathione Peroxidase 3 by Promoter Hypermethylation and Its Clinical Implication in Clear Cell Renal Cell Carcinoma
Previous Article in Special Issue
Cyanobacterial Hydrogenases and Hydrogen Metabolism Revisited: Recent Progress and Future Prospects
Open AccessArticle

Biohydrogen and Bioethanol Production from Biodiesel-Based Glycerol by Enterobacter aerogenes in a Continuous Stir Tank Reactor

Department of Chemical Engineering, McGill University, 3610 University Street, Montreal, QC H3A-0C5, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Patrick Hallenbeck
Int. J. Mol. Sci. 2015, 16(5), 10650-10664; https://doi.org/10.3390/ijms160510650
Received: 29 March 2015 / Revised: 2 May 2015 / Accepted: 5 May 2015 / Published: 11 May 2015
(This article belongs to the Special Issue Photosynthesis and Biological Hydrogen Production)
Crude glycerol from the biodiesel manufacturing process is being produced in increasing quantities due to the expanding number of biodiesel plants. It has been previously shown that, in batch mode, semi-anaerobic fermentation of crude glycerol by Enterobacter aerogenes can produce biohydrogen and bioethanol simultaneously. The present study demonstrated the possible scaling-up of this process from small batches performed in small bottles to a 3.6-L continuous stir tank reactor (CSTR). Fresh feed rate, liquid recycling, pH, mixing speed, glycerol concentration, and waste recycling were optimized for biohydrogen and bioethanol production. Results confirmed that E. aerogenes uses small amounts of oxygen under semi-anaerobic conditions for growth before using oxygen from decomposable salts, mainly NH4NO3, under anaerobic condition to produce hydrogen and ethanol. The optimal conditions were determined to be 500 rpm, pH 6.4, 18.5 g/L crude glycerol (15 g/L glycerol) and 33% liquid recycling for a fresh feed rate of 0.44 mL/min. Using these optimized conditions, the process ran at a lower media cost than previous studies, was stable after 7 days without further inoculation and resulted in yields of 0.86 mol H2/mol glycerol and 0.75 mol ethanol/mole glycerol. View Full-Text
Keywords: biohydrogen; bioethanol; crude glycerol; Enterobacter aerogenes; CSTR biohydrogen; bioethanol; crude glycerol; Enterobacter aerogenes; CSTR
Show Figures

Figure 1

MDPI and ACS Style

Jitrwung, R.; Yargeau, V. Biohydrogen and Bioethanol Production from Biodiesel-Based Glycerol by Enterobacter aerogenes in a Continuous Stir Tank Reactor. Int. J. Mol. Sci. 2015, 16, 10650-10664.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop