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The Potential of the Combination of CRISPR/Cas9 and Pluripotent Stem Cells to Provide Human Organs from Chimaeric Pigs

Shenzhen Key Laboratory of Xenotransplantaton, State and Local Joint Cancer Genome Clinical Application of Key Technology Laboratory, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen 518039, China
Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, Nanjing 210029, China
Thomas E. Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
State Key Laboratory of Conservation & Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530005, China
Authors to whom correspondence should be addressed.
Academic Editor: Izuho Hatada
Int. J. Mol. Sci. 2015, 16(3), 6545-6556;
Received: 28 January 2015 / Revised: 11 March 2015 / Accepted: 16 March 2015 / Published: 23 March 2015
(This article belongs to the Special Issue Genome Editing)
Clinical organ allotransplantation is limited by the availability of deceased human donors. However, the transplantation of human organs produced in other species would provide an unlimited number of organs. The pig has been identified as the most suitable source of organs for humans as organs of any size would be available. Genome editing by RNA-guided endonucleases, also known as clustered regularly interspaced short palindromic repeat (CRISPR/Cas9), in combination with induced pluripotent stem cells (iPSC), may have the potential to enable the creation of human organs from genetically-modified chimaeric pigs. These could potentially provide an unlimited supply of organs that would not be rejected by the recipient’s immune system. However, substantial research is needed to prove that this approach will work. Genetic modification of chimaeric pigs could also provide useful models for developing therapies for various human diseases, especially in relation to drug development. View Full-Text
Keywords: CRISPR/Cas9; genome editing; iPSC; pigs; chimaeric; xenotransplantation CRISPR/Cas9; genome editing; iPSC; pigs; chimaeric; xenotransplantation
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Feng, W.; Dai, Y.; Mou, L.; Cooper, D.K.C.; Shi, D.; Cai, Z. The Potential of the Combination of CRISPR/Cas9 and Pluripotent Stem Cells to Provide Human Organs from Chimaeric Pigs. Int. J. Mol. Sci. 2015, 16, 6545-6556.

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