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Open AccessArticle

Characterization and Antihyperglycemic Activity of a Polysaccharide from Dioscorea opposita Thunb Roots

by Yijun Fan 1,*,†, Qinyi He 1, Aoshuang Luo 2,†, Miaoyu Wang 1 and Aoxue Luo 1,*
1
Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China
2
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Brijesh Tiwari
Int. J. Mol. Sci. 2015, 16(3), 6391-6401; https://doi.org/10.3390/ijms16036391
Received: 29 January 2015 / Revised: 1 March 2015 / Accepted: 16 March 2015 / Published: 19 March 2015
(This article belongs to the Special Issue Bioactive Carbohydrates and Peptides)
A polysaccharide DOTP-80 from Dioscorea opposita Thunb was obtained by using the method of acid water-extraction and ethanol-precipitation. After being purified by chromatography, the structure characteristics of DOTP-80 were established. Based on the calibration curve obtained with standard dextrans, the molecular weight of the polysaccharide fraction DOTP-80 was calculated to be 123 kDa. The results of Infrared spectrum (FT-IR) indicated that the polysaccharide contained the α-configuration of sugar units. GC-MS analysis revealed that DOTP-80 was mainly composed of mannose and glucose. Alloxan-induced diabetic rats and mice models were developed to evaluate the in vivo hypoglycemic activity of the polysaccharide. The results indicated that a high dose DOTP-80 (400 mg/kg) had strong hypoglycemic activity. Moreover, DOTP-80 could increase the level of antioxidant enzymes (SOD) activity in alloxan-induced diabetic mice and stimulate an increase in glucose disposal in diabetic rats. Therefore, the polysaccharide DOTP-80 should be evaluated as a candidate for future studies on diabetes mellitus. View Full-Text
Keywords: Dioscorea opposita Thunb; antihyperglycemic; polysaccharide Dioscorea opposita Thunb; antihyperglycemic; polysaccharide
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Fan, Y.; He, Q.; Luo, A.; Wang, M.; Luo, A. Characterization and Antihyperglycemic Activity of a Polysaccharide from Dioscorea opposita Thunb Roots. Int. J. Mol. Sci. 2015, 16, 6391-6401.

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