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Open AccessArticle

Characterization and Antihyperglycemic Activity of a Polysaccharide from Dioscorea opposita Thunb Roots

by Yijun Fan 1,*,†, Qinyi He 1, Aoshuang Luo 2,†, Miaoyu Wang 1 and Aoxue Luo 1,*
Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Brijesh Tiwari
Int. J. Mol. Sci. 2015, 16(3), 6391-6401;
Received: 29 January 2015 / Revised: 1 March 2015 / Accepted: 16 March 2015 / Published: 19 March 2015
(This article belongs to the Special Issue Bioactive Carbohydrates and Peptides)
A polysaccharide DOTP-80 from Dioscorea opposita Thunb was obtained by using the method of acid water-extraction and ethanol-precipitation. After being purified by chromatography, the structure characteristics of DOTP-80 were established. Based on the calibration curve obtained with standard dextrans, the molecular weight of the polysaccharide fraction DOTP-80 was calculated to be 123 kDa. The results of Infrared spectrum (FT-IR) indicated that the polysaccharide contained the α-configuration of sugar units. GC-MS analysis revealed that DOTP-80 was mainly composed of mannose and glucose. Alloxan-induced diabetic rats and mice models were developed to evaluate the in vivo hypoglycemic activity of the polysaccharide. The results indicated that a high dose DOTP-80 (400 mg/kg) had strong hypoglycemic activity. Moreover, DOTP-80 could increase the level of antioxidant enzymes (SOD) activity in alloxan-induced diabetic mice and stimulate an increase in glucose disposal in diabetic rats. Therefore, the polysaccharide DOTP-80 should be evaluated as a candidate for future studies on diabetes mellitus. View Full-Text
Keywords: Dioscorea opposita Thunb; antihyperglycemic; polysaccharide Dioscorea opposita Thunb; antihyperglycemic; polysaccharide
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Fan, Y.; He, Q.; Luo, A.; Wang, M.; Luo, A. Characterization and Antihyperglycemic Activity of a Polysaccharide from Dioscorea opposita Thunb Roots. Int. J. Mol. Sci. 2015, 16, 6391-6401.

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