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Open AccessArticle

Chloroplast-Expressed MSI-99 in Tobacco Improves Disease Resistance and Displays Inhibitory Effect against Rice Blast Fungus

Faculty of Agronomy, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun 130118, China
Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363 Shengtai Street, Changchun 130033, China
College of Biological Sciences, China Agricultural University, No. 2 West Yuanmingyuan Road, Beijing 100094, China
Authors to whom correspondence should be addressed.
Academic Editor: Jan Schirawski
Int. J. Mol. Sci. 2015, 16(3), 4628-4641;
Received: 27 November 2014 / Revised: 16 February 2015 / Accepted: 16 February 2015 / Published: 2 March 2015
(This article belongs to the Special Issue Plant Microbe Interaction)
PDF [3953 KB, uploaded 2 March 2015]


Rice blast is a major destructive fungal disease that poses a serious threat to rice production and the improvement of blast resistance is critical to rice breeding. The antimicrobial peptide MSI-99 has been suggested as an antimicrobial peptide conferring resistance to bacterial and fungal diseases. Here, a vector harboring the MSI-99 gene was constructed and introduced into the tobacco chloroplast genome via particle bombardment. Transformed plants were obtained and verified to be homoplastomic by PCR and Southern hybridization. In planta assays demonstrated that the transgenic tobacco plants displayed an enhanced resistance to the fungal disease. The evaluation of the antimicrobial activity revealed that the crude protein extracts from the transgenic plants manifested an antimicrobial activity against E. coli, even after incubation at 120 °C for 20 min, indicating significant heat stability of MSI-99. More importantly, the MSI-99-containing protein extracts were firstly proved in vitro and in vivo to display significant suppressive effects on two rice blast isolates. These findings provide a strong basis for the development of new biopesticides to combat rice blast. View Full-Text
Keywords: antimicrobial peptide; chloroplast transformation; rice blast; tobacco antimicrobial peptide; chloroplast transformation; rice blast; tobacco

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Wang, Y.-P.; Wei, Z.-Y.; Zhang, Y.-Y.; Lin, C.-J.; Zhong, X.-F.; Wang, Y.-L.; Ma, J.-Y.; Ma, J.; Xing, S.-C. Chloroplast-Expressed MSI-99 in Tobacco Improves Disease Resistance and Displays Inhibitory Effect against Rice Blast Fungus. Int. J. Mol. Sci. 2015, 16, 4628-4641.

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