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Int. J. Mol. Sci. 2015, 16(2), 2893-2912;

Regulation of Human Adenovirus Alternative RNA Splicing by the Adenoviral L4-33K and L4-22K Proteins

Department of Medical Biochemistry and Microbiology, Science for Life Laboratory Uppsala University, Uppsala 75123, Sweden
Author to whom correspondence should be addressed.
Academic Editor: Akila Mayeda
Received: 22 December 2014 / Revised: 6 January 2015 / Accepted: 22 January 2015 / Published: 28 January 2015
(This article belongs to the Special Issue Pre-mRNA Splicing)
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Adenovirus makes extensive use of alternative RNA splicing to produce a complex set of spliced viral mRNAs. Studies aimed at characterizing the interactions between the virus and the host cell RNA splicing machinery have identified three viral proteins of special significance for the control of late viral gene expression: L4-33K, L4-22K, and E4-ORF4. L4-33K is a viral alternative RNA splicing factor that controls L1 alternative splicing via an interaction with the cellular protein kinases Protein Kinase A (PKA) and DNA-dependent protein kinase (DNA-PK). L4-22K is a viral transcription factor that also has been implicated in the splicing of a subset of late viral mRNAs. E4-ORF4 is a viral protein that binds the cellular protein phosphatase IIA (PP2A) and controls Serine/Arginine (SR)-rich protein activity by inducing SR protein dephosphorylation. The L4-33K, and most likely also the L4-22K protein, are highly phosphorylated in vivo. Here we will review the function of these viral proteins in the post-transcriptional control of adenoviral gene expression and further discuss the significance of potential protein kinases phosphorylating the L4-33K and/or L4-22K proteins. View Full-Text
Keywords: adenovirus; L4-33K; L4-22K; RNA splicing; SR proteins; PKA; DNA-PK adenovirus; L4-33K; L4-22K; RNA splicing; SR proteins; PKA; DNA-PK

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Biasiotto, R.; Akusjärvi, G. Regulation of Human Adenovirus Alternative RNA Splicing by the Adenoviral L4-33K and L4-22K Proteins. Int. J. Mol. Sci. 2015, 16, 2893-2912.

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