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Int. J. Mol. Sci. 2015, 16(12), 30061-30074;

Biochemical Characterization of An Arginine-Specific Alkaline Trypsin from Bacillus licheniformis

School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Ritva Tikkanen
Received: 8 November 2015 / Revised: 8 December 2015 / Accepted: 9 December 2015 / Published: 17 December 2015
(This article belongs to the Section Biochemistry)
Full-Text   |   PDF [2231 KB, uploaded 17 December 2015]   |  


In the present study, we isolated a trypsin-producing strain DMN6 from the leather waste and identified it as Bacillus licheniformis through a two-step screening strategy. The trypsin activity was increased up to 140 from 20 U/mL through culture optimization. The enzyme was purified to electrophoretic homogeneity with a molecular mass of 44 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the specific activity of purified enzyme is 350 U/mg with Nα-Benzoyl-l-arginine ethylester as the substrate. The optimum temperature and pH for the trypsin are 65 °C and pH 9.0, respectively. Also, the enzyme can be significantly activated by Ba2+. This enzyme is relatively stable in alkaline environment and displays excellent activity at low temperatures. It could retain over 95% of enzyme activity after 180 min of incubation at 45 °C. The distinguished activity under low temperature and prominent stability enhance its catalytic potential. In the current work, the open reading frame was obtained with a length of 1371 nucleotides that encoded a protein of 456 amino acids. These data would warrant the B. licheniformis trypsin as a promising candidate for catalytic application in collagen preparation and leather bating through further protein engineering. View Full-Text
Keywords: trypsin; Bacillus licheniformis; enzymatic properties; cloning; biocatalysis trypsin; Bacillus licheniformis; enzymatic properties; cloning; biocatalysis

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Gong, J.-S.; Li, W.; Zhang, D.-D.; Xie, M.-F.; Yang, B.; Zhang, R.-X.; Li, H.; Lu, Z.-M.; Xu, Z.-H.; Shi, J.-S. Biochemical Characterization of An Arginine-Specific Alkaline Trypsin from Bacillus licheniformis. Int. J. Mol. Sci. 2015, 16, 30061-30074.

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