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Int. J. Mol. Sci. 2015, 16(12), 28311-28319;

RpoN Regulates Virulence Factors of Pseudomonas aeruginosa via Modulating the PqsR Quorum Sensing Regulator

Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551
Interdisciplinary Graduate School, Nanyang Technological University, Singapore 637551
Department of Respiratory Disease, First Affiliated Hospital of Guangxi Medical University, Nanning 530000, China
Costerton Biofilm Center, Department of International Health, Immunology and Microbiology, University of Copenhagen, 2200 København N, Denmark
School of Biological Sciences, Nanyang Technological University, Singapore 637551
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Nicholas Delihas
Received: 13 August 2015 / Revised: 9 November 2015 / Accepted: 23 November 2015 / Published: 30 November 2015
(This article belongs to the Section Biochemistry)
Full-Text   |   PDF [3308 KB, uploaded 30 November 2015]   |  


The alternative sigma factor RpoN regulates many cell functions, such as motility, quorum sensing, and virulence in the opportunistic pathogen Pseudomonas aeruginosa (P. aeruginosa). P. aeruginosa often evolves rpoN-negative variants during the chronic infection in cystic fibrosis patients. It is unclear how RpoN interacts with other regulatory mechanisms to control virulence of P. aeruginosa. In this study, we show that RpoN modulates the function of PqsR, a quorum sensing receptor regulating production of virulence factors including the phenazine pyocyanin. The ∆rpoN mutant is able to synthesize 4-quinolone signal molecule HHQ but unable to activate PqsR and Pseudomonas quinolone signal (pqs) quorum sensing. The ∆rpoN mutant produces minimal level of pyocyanin and is unable to produce the anti-staphylococcal agents. Providing pqsR in trans in the ∆rpoN mutant restores its pqs quorum sensing and virulence factor production to the wild-type level. Our study provides evidence that RpoN has a regulatory effect on P. aeruginosa virulence through modulating the function of the PqsR quorum sensing regulator. View Full-Text
Keywords: Pseudomonas aeruginosa; rpoN; pqsR; quorum sensing Pseudomonas aeruginosa; rpoN; pqsR; quorum sensing

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Cai, Z.; Liu, Y.; Chen, Y.; Yam, J.K.H.; Chew, S.C.; Chua, S.L.; Wang, K.; Givskov, M.; Yang, L. RpoN Regulates Virulence Factors of Pseudomonas aeruginosa via Modulating the PqsR Quorum Sensing Regulator. Int. J. Mol. Sci. 2015, 16, 28311-28319.

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