Next Article in Journal
Cell-Penetrating Ability of Peptide Hormones: Key Role of Glycosaminoglycans Clustering
Next Article in Special Issue
Protective Effects of Cardamom in Isoproterenol-Induced Myocardial Infarction in Rats
Previous Article in Journal
The Correlation between Chitin and Acidic Mammalian Chitinase in Animal Models of Allergic Asthma
Previous Article in Special Issue
Exploiting the Pleiotropic Antioxidant Effects of Established Drugs in Cardiovascular Disease
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessReview
Int. J. Mol. Sci. 2015, 16(11), 27378-27390;

Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies

1,†,* and 2,†
Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands
Faculty of Medical Sciences, Goce Delcev University, 2000 Stip, Macedonia
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Guido R. M. M. Haenen
Received: 19 September 2015 / Revised: 30 October 2015 / Accepted: 2 November 2015 / Published: 16 November 2015
(This article belongs to the Special Issue Antioxidant 2.0——Redox Modulation by Food and Drugs)
Full-Text   |   PDF [1718 KB, uploaded 16 November 2015]   |  


In this review, we disclose a selection of serum/plasma biomarkers of (anti)oxidant status related to nutrition, which can be used for measurements in large-scale epidemiological studies. From personal experience, we have come to the following proposal of a set of biomarkers for nutritional intake, (anti)oxidant status, and redox status. We have selected the individual antioxidant vitamins E and A, and the carotenoids which can be measured in large series by HPLC. In addition, vitamin C was selected, which can be measured by an auto-analyzer or HPLC. As a biomarker for oxidative stress, the ROM assay (reactive oxygen metabolites) was selected; for the redox status, the total thiol assay; and for the total antioxidant status the BAP assay (biological antioxidant potential). All of these biomarkers can be measured in large quantities by an auto-analyzer. Critical points in biomarker validation with respect to blood sampling, storage conditions, and measurements are discussed. With the selected biomarkers, a good set is presented for use in the risk assessment between nutrition and (chronic) diseases in large-scale epidemiological studies. Examples of the successful application of these biomarkers in large international studies are presented. View Full-Text
Keywords: biomarkers; nutrition; vitamins; oxidative stress; antioxidant status; redox status; epidemiology biomarkers; nutrition; vitamins; oxidative stress; antioxidant status; redox status; epidemiology

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Jansen, E.; Ruskovska, T. Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies. Int. J. Mol. Sci. 2015, 16, 27378-27390.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top