A PPO Promoter from Betalain-Producing Red Swiss Chard, Directs Petiole- and Root-Preferential Expression of Foreign Gene in Anthocyanins-Producing Plants
Abstract
:1. Introduction
2. Results
2.1. Cloning and Sequence Analysis of the BvcPPOP Promoter
2.2. BvcPPOP Directed Petiole- and Root-Preferential Expression of GUS Gene at the Vegetative Stage of Transgenic Arabidopsis
2.3. BvcPPOP-Directed GUS Expression Is Regulated by Developmental Stages
2.4. BvcPPOP-Directed GUS Expression Is Differently Regulated by Abiotic Stressors
2.5. 5′-Truncated BvcPPOPs Display Various Expression Patterns and/or Levels in Transgenic Arabidopsis
3. Discussion
4. Experimental Section
4.1. Plant Materials and Growth Conditions
4.2. Isolation of the Promoter and Its Deletion Analysis
Primer Name | Primer Sequence (5′ to 3′) * |
---|---|
Forward | |
FW1 | AAGCTTGCAAATGGTCTATATCCGAC |
FW2 | AAGCTTGAGATAAACTCGATCAAGCTCG |
FW3 | AAGCTTGAGTAGTAGCAGTACAAGTTG |
FW4 | AAGCTTAGTATGTGTATGTCTTGAGTG |
FW5 | AAGCTTTACTAGTCGTAAAGTAGCAGTTCTC |
Reverse | |
RW | GGATCCTCTTTTATATATTTGGTGGA |
4.3. Plant Expression Vector Construction and Agrobacterium-Mediated Transformation
4.4. Analysis of GUS Expression
4.5. Abiotic Stress Treatment
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Yu, Z.-H.; Han, Y.-N.; Xiao, X.-G. A PPO Promoter from Betalain-Producing Red Swiss Chard, Directs Petiole- and Root-Preferential Expression of Foreign Gene in Anthocyanins-Producing Plants. Int. J. Mol. Sci. 2015, 16, 27032-27043. https://doi.org/10.3390/ijms161126011
Yu Z-H, Han Y-N, Xiao X-G. A PPO Promoter from Betalain-Producing Red Swiss Chard, Directs Petiole- and Root-Preferential Expression of Foreign Gene in Anthocyanins-Producing Plants. International Journal of Molecular Sciences. 2015; 16(11):27032-27043. https://doi.org/10.3390/ijms161126011
Chicago/Turabian StyleYu, Zhi-Hai, Ya-Nan Han, and Xing-Guo Xiao. 2015. "A PPO Promoter from Betalain-Producing Red Swiss Chard, Directs Petiole- and Root-Preferential Expression of Foreign Gene in Anthocyanins-Producing Plants" International Journal of Molecular Sciences 16, no. 11: 27032-27043. https://doi.org/10.3390/ijms161126011