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Numerical Analysis of Hydrodynamic Flow in Microfluidic Biochip for Single-Cell Trapping Application

Department of Control and Mechatronic Engineering, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, Skudai, Johor 81310, Malaysia
Institute of Ibnu Sina, Universiti Teknologi Malaysia, Skudai, Johor 81310, Malaysia
Author to whom correspondence should be addressed.
Academic Editor: Fan-Gang Tseng
Int. J. Mol. Sci. 2015, 16(11), 26770-26785;
Received: 30 May 2015 / Revised: 29 July 2015 / Accepted: 5 August 2015 / Published: 9 November 2015
(This article belongs to the Special Issue Single Cell Analysis in Biotechnology and Systems Biology)
PDF [5358 KB, uploaded 9 November 2015]


Single-cell analysis has become the interest of a wide range of biological and biomedical engineering research. It could provide precise information on individual cells, leading to important knowledge regarding human diseases. To perform single-cell analysis, it is crucial to isolate the individual cells before further manipulation is carried out. Recently, microfluidic biochips have been widely used for cell trapping and single cell analysis, such as mechanical and electrical detection. This work focuses on developing a finite element simulation model of single-cell trapping system for any types of cells or particles based on the hydrodynamic flow resistance (Rh) manipulations in the main channel and trap channel to achieve successful trapping. Analysis is carried out using finite element ABAQUS-FEA™ software. A guideline to design and optimize single-cell trapping model is proposed and the example of a thorough optimization analysis is carried out using a yeast cell model. The results show the finite element model is able to trap a single cell inside the fluidic environment. Fluid’s velocity profile and streamline plots for successful and unsuccessful single yeast cell trapping are presented according to the hydrodynamic concept. The single-cell trapping model can be a significant important guideline in designing a new chip for biomedical applications. View Full-Text
Keywords: microfluidic; hydrodynamic; single-cell trapping; finite element microfluidic; hydrodynamic; single-cell trapping; finite element

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Khalili, A.A.; Ahmad, M.R. Numerical Analysis of Hydrodynamic Flow in Microfluidic Biochip for Single-Cell Trapping Application. Int. J. Mol. Sci. 2015, 16, 26770-26785.

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