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Int. J. Mol. Sci. 2015, 16(11), 26077-26086;

Application of CRISPR/Cas9 Technology to HBV

National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China
Author to whom correspondence should be addressed.
Academic Editor: Izuho Hatada
Received: 8 September 2015 / Revised: 25 October 2015 / Accepted: 26 October 2015 / Published: 2 November 2015
(This article belongs to the Special Issue Genome Editing)
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More than 240 million people around the world are chronically infected with hepatitis B virus (HBV). Nucleos(t)ide analogs and interferon are the only two families of drugs to treat HBV currently. However, none of these anti-virals directly target the stable nuclear covalently closed circular DNA (cccDNA), which acts as a transcription template for viral mRNA and pre-genomic RNA synthesis and secures virus persistence. Thus, the fact that only a small number of patients treated achieve sustained viral response (SVR) or cure, highlights the need for new therapies against HBV. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing system can specifically target the conserved regions of the HBV genome. This results in robust viral suppression and provides a promising tool for eradicating the virus. In this review, we discuss the function and application of the CRISPR/Cas9 system as a novel therapy for HBV. View Full-Text
Keywords: CRISPR/Cas9; HBV; cccDNA; antiviral CRISPR/Cas9; HBV; cccDNA; antiviral

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Lin, G.; Zhang, K.; Li, J. Application of CRISPR/Cas9 Technology to HBV. Int. J. Mol. Sci. 2015, 16, 26077-26086.

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