Int. J. Mol. Sci. 2015, 16(11), 25982-25998; https://doi.org/10.3390/ijms161125934
Proteomics Analysis of Cellular Proteins Co-Immunoprecipitated with Nucleoprotein of Influenza A Virus (H7N9)
1
Central Laboratory, Jilin University Second Hospital, Changchun 130041, China
2
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China
3
College of Life Sciences, Shenzhen University, Shenzhen 518057, China
â€
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: David Sheehan
Received: 26 August 2015 / Revised: 13 October 2015 / Accepted: 22 October 2015 / Published: 30 October 2015
(This article belongs to the Collection Advances in Proteomic Research)
Abstract
Avian influenza A viruses are serious veterinary pathogens that normally circulate among avian populations, causing substantial economic impacts. Some strains of avian influenza A viruses, such as H5N1, H9N2, and recently reported H7N9, have been occasionally found to adapt to humans from other species. In order to replicate efficiently in the new host, influenza viruses have to interact with a variety of host factors. In the present study, H7N9 nucleoprotein was transfected into human HEK293T cells, followed by immunoprecipitated and analyzed by proteomics approaches. A series of host proteins co-immunoprecipitated were identified with high confidence, some of which were found to be acetylated at their lysine residues. Bioinformatics analysis revealed that spliceosome might be the most relevant pathway involved in host response to nucleoprotein expression, increasing our emerging knowledge of host proteins that might be involved in influenza virus replication activities. View Full-TextKeywords:
nucleoprotein; influenza A virus; lysine acetylation; mass spectrometry; co-immunoprecipitation
▼
Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Share & Cite This Article
MDPI and ACS Style
Sun, N.; Sun, W.; Li, S.; Yang, J.; Yang, L.; Quan, G.; Gao, X.; Wang, Z.; Cheng, X.; Li, Z.; Peng, Q.; Liu, N. Proteomics Analysis of Cellular Proteins Co-Immunoprecipitated with Nucleoprotein of Influenza A Virus (H7N9). Int. J. Mol. Sci. 2015, 16, 25982-25998.
Related Articles
Article Metrics
Comments
[Return to top]
Int. J. Mol. Sci.
EISSN 1422-0067
Published by MDPI AG, Basel, Switzerland
RSS
E-Mail Table of Contents Alert