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Genome Editing Using Mammalian Haploid Cells

Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, Gunma 371-8512, Japan
Author to whom correspondence should be addressed.
Academic Editor: Mark Richter
Int. J. Mol. Sci. 2015, 16(10), 23604-23614;
Received: 14 August 2015 / Revised: 14 September 2015 / Accepted: 28 September 2015 / Published: 1 October 2015
(This article belongs to the Special Issue Genome Editing)
Haploid cells are useful for studying gene functions because disruption of a single allele can cause loss-of-function phenotypes. Recent success in generating haploid embryonic stem cells (ESCs) in mice, rats, and monkeys provides a new platform for simple genetic manipulation of the mammalian genome. Use of haploid ESCs enhances the genome-editing potential of the CRISPR/Cas system. For example, CRISPR/Cas was used in haploid ESCs to generate multiple knockouts and large deletions at high efficiency. In addition, genome-wide screening is facilitated by haploid cell lines containing gene knockout libraries. View Full-Text
Keywords: haploid; embryonic stem cell; CRISPR/Cas haploid; embryonic stem cell; CRISPR/Cas
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Horii, T.; Hatada, I. Genome Editing Using Mammalian Haploid Cells. Int. J. Mol. Sci. 2015, 16, 23604-23614.

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