Next Article in Journal
Mycophenolate Antagonizes IFN-γ-Induced Catagen-Like Changes via β-Catenin Activation in Human Dermal Papilla Cells and Hair Follicles
Next Article in Special Issue
Caveolin-1 Limits the Contribution of BKCa Channel to MCF-7 Breast Cancer Cell Proliferation and Invasion
Previous Article in Journal
Electricity Generation and Wastewater Treatment of Oil Refinery in Microbial Fuel Cells Using Pseudomonas putida
Previous Article in Special Issue
PL-PatchSurfer: A Novel Molecular Local Surface-Based Method for Exploring Protein-Ligand Interactions
Article

Metabolism of Cryptic Peptides Derived from Neuropeptide FF Precursors: The Involvement of Insulin-Degrading Enzyme

1
Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy
2
Department of Biochemistry and Neurobiology, AGH University of Science and Technology, Mickiewicza 30, 30-059 Krakow, Poland
3
Centre for Polymer and Carbon Materials, Polish Academy of Sciences, Sklodowskiej-Curie 34, 41-819 Zabrze, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(9), 16787-16799; https://doi.org/10.3390/ijms150916787
Received: 5 June 2014 / Revised: 3 September 2014 / Accepted: 9 September 2014 / Published: 22 September 2014
(This article belongs to the Collection Proteins and Protein-Ligand Interactions)
The term “cryptome” refers to the subset of cryptic peptides with bioactivities that are often unpredictable and very different from the parent protein. These cryptic peptides are generated by proteolytic cleavage of proteases, whose identification in vivo can be very challenging. In this work, we show that insulin-degrading enzyme (IDE) is able to degrade specific amino acid sequences present in the neuropeptide pro-NPFFA (NPFF precursor), generating some cryptic peptides that are also observed after incubation with rat brain cortex homogenate. The reported experimental findings support the increasingly accredited hypothesis, according to which, due to its wide substrate selectivity, IDE is involved in a wide variety of physiopathological processes. View Full-Text
Keywords: IDE; neuropeptide; metalloprotease; cryptome; mass spectrometry; surface plasmon resonance IDE; neuropeptide; metalloprotease; cryptome; mass spectrometry; surface plasmon resonance
Show Figures

Graphical abstract

MDPI and ACS Style

Grasso, G.; Mielczarek, P.; Niedziolka, M.; Silberring, J. Metabolism of Cryptic Peptides Derived from Neuropeptide FF Precursors: The Involvement of Insulin-Degrading Enzyme. Int. J. Mol. Sci. 2014, 15, 16787-16799. https://doi.org/10.3390/ijms150916787

AMA Style

Grasso G, Mielczarek P, Niedziolka M, Silberring J. Metabolism of Cryptic Peptides Derived from Neuropeptide FF Precursors: The Involvement of Insulin-Degrading Enzyme. International Journal of Molecular Sciences. 2014; 15(9):16787-16799. https://doi.org/10.3390/ijms150916787

Chicago/Turabian Style

Grasso, Giuseppe, Przemyslaw Mielczarek, Magdalena Niedziolka, and Jerzy Silberring. 2014. "Metabolism of Cryptic Peptides Derived from Neuropeptide FF Precursors: The Involvement of Insulin-Degrading Enzyme" International Journal of Molecular Sciences 15, no. 9: 16787-16799. https://doi.org/10.3390/ijms150916787

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop