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Open AccessArticle

In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine

1
Department of Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, PO Box 9530, Morgantown, WV 26506, USA
2
Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506, USA
*
Author to whom correspondence should be addressed.
Current address: Memorial Sloan Kettering Cancer Center, Molecular Pharmacology and Chemistry, 1275 York Avenue, Box 425, New York, NY 10065, USA.
Int. J. Mol. Sci. 2014, 15(8), 14332-14347; https://doi.org/10.3390/ijms150814332
Received: 5 June 2014 / Revised: 11 July 2014 / Accepted: 8 August 2014 / Published: 18 August 2014
(This article belongs to the Section Molecular Recognition)
Widespread use of the chlorotriazine herbicide, atrazine, has led to serious environmental and human health consequences. Current methods of detecting atrazine contamination are neither rapid nor cost-effective. In this work, atrazine-specific single-stranded DNA (ssDNA) molecular recognition elements (MRE) were isolated. We utilized a stringent Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology that placed the greatest emphasis on what the MRE should not bind to. After twelve rounds of SELEX, an atrazine-specific MRE with high affinity was obtained. The equilibrium dissociation constant (Kd) of the ssDNA sequence is 0.62 ± 0.21 nM. It also has significant selectivity for atrazine over atrazine metabolites and other pesticides found in environmentally similar locations and concentrations. Furthermore, we have detected environmentally relevant atrazine concentrations in river water using this MRE. The strong affinity and selectivity of the selected atrazine-specific ssDNA validated the stringent SELEX methodology and identified a MRE that will be useful for rapid atrazine detection in environmental samples. View Full-Text
Keywords: SELEX; in vitro selection; aptamer; molecular recognition element (MRE); atrazine SELEX; in vitro selection; aptamer; molecular recognition element (MRE); atrazine
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MDPI and ACS Style

Williams, R.M.; Crihfield, C.L.; Gattu, S.; Holland, L.A.; Sooter, L.J. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. Int. J. Mol. Sci. 2014, 15, 14332-14347. https://doi.org/10.3390/ijms150814332

AMA Style

Williams RM, Crihfield CL, Gattu S, Holland LA, Sooter LJ. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. International Journal of Molecular Sciences. 2014; 15(8):14332-14347. https://doi.org/10.3390/ijms150814332

Chicago/Turabian Style

Williams, Ryan M.; Crihfield, Cassandra L.; Gattu, Srikanth; Holland, Lisa A.; Sooter, Letha J. 2014. "In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine" Int. J. Mol. Sci. 15, no. 8: 14332-14347. https://doi.org/10.3390/ijms150814332

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