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Open AccessArticle

Effects of Pulsed Electromagnetic Field on Differentiation of HUES-17 Human Embryonic Stem Cell Line

1
Department of Radiation Medicine, the Fourth Military Medical University, Xi'an 710032, China
2
Experiment Teaching Center, the Fourth Military Medical University, Xi'an 710032, China
3
Department of Pathology and Pathophysiology, the Fourth Military Medical University, Xi'an 710032, China
4
Department of Neurosurgery, the First Hospital of Jilin University, Changchun 130021, China
5
Department of Pharmaceutics, the Fourth Military Medical University, Xi'an 710032, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2014, 15(8), 14180-14190; https://doi.org/10.3390/ijms150814180
Received: 11 June 2014 / Revised: 14 July 2014 / Accepted: 25 July 2014 / Published: 14 August 2014
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Electromagnetic fields are considered to potentially affect embryonic development, but the mechanism is still unknown. In this study, human embryonic stem cell (hESC) line HUES-17 was applied to explore the mechanism of exposure on embryonic development to pulsed electromagnetic field (PEMF) for 400 pulses at different electric field intensities and the differentiation of HUES-17 cells was observed after PEMF exposure. The expression of alkaline phosphatase (AP), stage-specific embryonic antigen-3 (SSEA-3), SSEA-4 and the mRNA level and protein level of Oct4, Sox2 and Nanog in HUES-17 cells remained unchanged after PEMF exposure at the electric field intensities of 50, 100, 200 or 400 kV/m. Four hundred pulses PEMF exposure at the electric field intensities of 50, 100, 200 or 400 kV/m did not affect the differentiation of HUES-17 cells. The reason why electromagnetic fields affect embryonic development may be due to other mechanisms rather than affecting the differentiation of embryonic stem cells. View Full-Text
Keywords: PEMF; hESC; differentiation PEMF; hESC; differentiation
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MDPI and ACS Style

Wu, Y.-L.; Ma, S.-R.; Peng, T.; Teng, Z.-H.; Liang, X.-Y.; Guo, G.-Z.; Zhang, H.-F.; Li, K.-C. Effects of Pulsed Electromagnetic Field on Differentiation of HUES-17 Human Embryonic Stem Cell Line. Int. J. Mol. Sci. 2014, 15, 14180-14190.

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