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Int. J. Mol. Sci. 2014, 15(7), 13123-13134;

Crosslinking Liposomes/Cells Using Cholesteryl Group-Modified Tilapia Gelatin

Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
Author to whom correspondence should be addressed.
Received: 1 May 2014 / Revised: 14 July 2014 / Accepted: 15 July 2014 / Published: 23 July 2014
(This article belongs to the Special Issue Biodegradable Materials)
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Cholesteryl group-modified tilapia gelatins (Chol-T-Gltns) with various Chol contents from 3 to 69 mol % per amino group of Gltn were prepared for the assembly of liposomes and cells. Liposomes were physically crosslinked by anchoring Chol groups of Chol-T-Gltns into lipid membranes. The resulting liposome gels were enzymatically degraded by addition of collagenase. Liposome gels prepared using Chol-T-Gltn with high Chol content (69Chol-T-Gltn) showed slower enzymatic degradation when compared with gels prepared using Chol-T-Gltn with low Chol content (3Chol-T-Gltn). The hepatocyte cell line HepG2 showed good assembly properties and no cytotoxic effects after addition of 69Chol-T-Gltns. In addition, the number of HepG2 cells increased with concentration of 69Chol-T-Gltns. Therefore, Chol-T-Gltn, particularly, 69Chol-T-Gltn, can be used as an assembling material for liposomes and various cell types. The resulting organization can be applied to various biomedical fields, such as drug delivery systems, tissue engineering and regenerative medicine. View Full-Text
Keywords: hydrophobically modified gelatin; cholesterol; assembly; liposome; cell hydrophobically modified gelatin; cholesterol; assembly; liposome; cell

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Taguchi, T.; Endo, Y. Crosslinking Liposomes/Cells Using Cholesteryl Group-Modified Tilapia Gelatin. Int. J. Mol. Sci. 2014, 15, 13123-13134.

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