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Int. J. Mol. Sci. 2014, 15(6), 9481-9496;

A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma

Department of Lymphoma, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China
Department of Blood Transfusion, Jinan Military General Hospital, Jinan 250031, China
Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin Shengfa NabioTech Co., Ltd., Tianjin 300457, China
Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China
Division of Science Popularization, China Science and Technology Exchange Center, Beijing 100045, China
Authors to whom correspondence should be addressed.
Received: 21 April 2014 / Revised: 13 May 2014 / Accepted: 14 May 2014 / Published: 28 May 2014
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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Objective: To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody. Methods: Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA). Results: We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro. Conclusion: The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies. View Full-Text
Keywords: immune VHH phage display library; B cell-activating factor; sdAb immune VHH phage display library; B cell-activating factor; sdAb

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Wu, W.; Li, S.; Zhang, W.; Sun, J.; Ren, G.; Dong, Q. A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma. Int. J. Mol. Sci. 2014, 15, 9481-9496.

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