Brain Metastases from Lung Cancer Show Increased Expression of DVL1, DVL3 and Beta-Catenin and Down-Regulation of E-Cadherin
Abstract
:1. Introduction
2. Results
Patient No. | Location | CDH1 D16S752&D16S265&D16S398 | E-Cadherin | Beta-Catenin | Dishevelled-1 | Dishevelled-3 | Primary Tumor |
---|---|---|---|---|---|---|---|
1 | Cerebellum | LOH D16S265 | 0 | C++ N+ | ++ | +++ | Large cell carcinoma |
2 | Cerebellum | HETERO | +++ | C++ | ++ | ++ | Large cell carcinoma |
3 | Frontal region | HETERO | ++ | 0 | + | ++ | Large cell carcinoma |
4 | Parietal region | HETERO | +++ | C+ | +++ | ++ | Large cell carcinoma |
5 | Occipital region | HETERO | + | C++ | ++ | +++ | Large cell carcinoma |
6 | Frontal region | HETERO | + | N++ | ++ | + | Large cell carcinoma |
7 | Occipital region | HETERO | ++ | C+N+++ | ++ | ++ | Large cell carcinoma |
8 | Frontal region | HETERO | + | C++ | + | ++ | Large cell carcinoma |
9 | Parietal region | HETERO | + | C+ | ++ | ++ | SCLC |
10 | Frontal region | LOH D16S398 | 0 | C+ | ++ | ++ | SCLC |
11 | Parietal region | LOH all | 0 | C++N+++ | ++ | ++ | SCLC |
12 | Parietooccipital region | LOH D16S265 | ++ | C+ | +++ | ++ | SCLC |
13 | Parietal | ND | ND | ND | ++ | ++ | SCLC |
14 | Parietal | ND | ND | ND | + | + | SCLC |
15 | Cerebellum | ND | ND | ND | +++ | ++ | SCLC |
16 | Cerebellum | MSI D16S265 | 0 | C+ | +++ | +++ | Adenocarcinoma |
17 | Temporal region | HETERO | + | C+ | ++ | ++ | Adenocarcinoma |
18 | Parietal region | LOH D16S752 | ++ | C+ | +++ | +++ | Adenocarcinoma |
19 | PRFrontal region | NDLOH all | ++++++ | C+++C++N+ | ++++ | ++++ | Adenocarcinoma |
20 | Temporal region | LOH D16S398 | ++ | C+ | ++ | ++ | Adenocarcinoma |
21 | PRCerebellum | NDLOH D16S752 | ++++ | 0C+ | +++++ | ++++ | Adenocarcinoma |
22 | Temporal | ND | ND | ND | +++ | + | Adenocarcinoma |
23 | Frontoparietal | ND | ND | ND | ++ | ++ | Adenocarcinoma |
24 | Frontoparietal | ND | ND | ND | +++ | ++ | Adenocarcinoma |
25 | Parietal region | LOH D16S398 | 0 | C++ | + | +++ | Squamous cell carcinoma |
26 | Parietal region | HETERO | +++ | C+N++ | +++ | +++ | Squamous cell carcinoma |
27 | Parietal region | HETERO | +++ | C+++ | ++ | +++ | Squamous cell carcinoma |
28 | Multiple metastases | HETERO | ++ | C++N+ | ++ | +++ | Squamous cell carcinoma |
29 | Cerebellum | HETERO | + | C+N++ | +++ | +++ | Squamous cell carcinoma |
30 | PRTemporal region | NDHETERO | ++++ | C++C+++N+ | +++++ | ++++++ | Carcinosarcoma |
31 | PRParietal region | NDHETERO | ++++ | 0C+ | ++++ | +++++ | Carcinosarcoma |
3. Discussion
4. Experimental Section
4.1. Tumor Specimen
4.2. Immunohistochemistry
4.3. Genetic Analyses of E-Cadherin and Beta-Catenin
4.3.1. Polymerase Chain Reaction (PCR)
4.3.2. Loss of Heterozygosity, Microsatellite Instability (MSI)
4.3.3. Heteroduplex Analysis
4.4. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Kafka, A.; Tomas, D.; Beroš, V.; Pećina, H.I.; Zeljko, M.; Pećina-Šlaus, N. Brain Metastases from Lung Cancer Show Increased Expression of DVL1, DVL3 and Beta-Catenin and Down-Regulation of E-Cadherin. Int. J. Mol. Sci. 2014, 15, 10635-10651. https://doi.org/10.3390/ijms150610635
Kafka A, Tomas D, Beroš V, Pećina HI, Zeljko M, Pećina-Šlaus N. Brain Metastases from Lung Cancer Show Increased Expression of DVL1, DVL3 and Beta-Catenin and Down-Regulation of E-Cadherin. International Journal of Molecular Sciences. 2014; 15(6):10635-10651. https://doi.org/10.3390/ijms150610635
Chicago/Turabian StyleKafka, Anja, Davor Tomas, Vili Beroš, Hrvoje Ivan Pećina, Martina Zeljko, and Nives Pećina-Šlaus. 2014. "Brain Metastases from Lung Cancer Show Increased Expression of DVL1, DVL3 and Beta-Catenin and Down-Regulation of E-Cadherin" International Journal of Molecular Sciences 15, no. 6: 10635-10651. https://doi.org/10.3390/ijms150610635
APA StyleKafka, A., Tomas, D., Beroš, V., Pećina, H. I., Zeljko, M., & Pećina-Šlaus, N. (2014). Brain Metastases from Lung Cancer Show Increased Expression of DVL1, DVL3 and Beta-Catenin and Down-Regulation of E-Cadherin. International Journal of Molecular Sciences, 15(6), 10635-10651. https://doi.org/10.3390/ijms150610635