Antenna-Specific Glutathione S-Transferase in Male Silkmoth Bombyx mori
Abstract
:1. Introduction
2. Results
2.1. Cloning and Analysis of Bmgstd4
2.2. Phylogenetic Analysis of BmGSTD4 with Other Insect GSTs
2.3. Structural Characterization of BmGSTD4
2.4. Antenna-Specific of BmGSTD4
2.5. Enzymatic Properties and GST Activity of BmGSTD4
3. Discussion
4. Experimental Section
4.1. Materials and Tissue Collection
4.2. Extraction of RNA and Synthesis of First-Strand cDNA
4.3. RT-PCR
4.4. Rapid Amplification of cDNA Ends of Bmgstd4
4.5. Sequence Analysis
4.6. Phylogenetic Analysis
4.7. Homology Modeling of BmGSTD4
4.8. Expression and Purification of Recombinant BmGSTD4
4.9. Western Blotting and Immunochemistry
4.10. Enzymatic Assays
5. Conclusions
Acknowledgments
Conflicts of Interest
References
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Tan, X.; Hu, X.-M.; Zhong, X.-W.; Chen, Q.-M.; Xia, Q.-Y.; Zhao, P. Antenna-Specific Glutathione S-Transferase in Male Silkmoth Bombyx mori. Int. J. Mol. Sci. 2014, 15, 7429-7443. https://doi.org/10.3390/ijms15057429
Tan X, Hu X-M, Zhong X-W, Chen Q-M, Xia Q-Y, Zhao P. Antenna-Specific Glutathione S-Transferase in Male Silkmoth Bombyx mori. International Journal of Molecular Sciences. 2014; 15(5):7429-7443. https://doi.org/10.3390/ijms15057429
Chicago/Turabian StyleTan, Xiang, Xiao-Ming Hu, Xiao-Wu Zhong, Quan-Mei Chen, Qing-You Xia, and Ping Zhao. 2014. "Antenna-Specific Glutathione S-Transferase in Male Silkmoth Bombyx mori" International Journal of Molecular Sciences 15, no. 5: 7429-7443. https://doi.org/10.3390/ijms15057429