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Large-Scale Domain Motions and Pyridoxal-5'-Phosphate Assisted Radical Catalysis in Coenzyme B12-Dependent Aminomutases

Physics Department, National Dong Hwa University, Hualien 97401, Taiwan
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Author to whom correspondence should be addressed.
This paper is dedicated to Emeritus Professor Perry A. Frey (Biochemistry Department, University of Wisconsin at Madison, Madison, WI, USA) on the occasion of his 78th birthday.
Int. J. Mol. Sci. 2014, 15(2), 3064-3087; https://doi.org/10.3390/ijms15023064
Received: 27 November 2013 / Revised: 25 December 2013 / Accepted: 22 January 2014 / Published: 20 February 2014
Lysine 5,6-aminomutase (5,6-LAM) and ornithine 4,5-aminomutase (4,5-OAM) are two of the rare enzymes that use assistance of two vitamins as cofactors. These enzymes employ radical generating capability of coenzyme B12 (5'-deoxyadenosylcobalamin, dAdoCbl) and ability of pyridoxal-5'-phosphate (PLP, vitamin B6) to stabilize high-energy intermediates for performing challenging 1,2-amino rearrangements between adjacent carbons. A large-scale domain movement is required for interconversion between the catalytically inactive open form and the catalytically active closed form. In spite of all the similarities, these enzymes differ in substrate specificities. 4,5-OAM is highly specific for D-ornithine as a substrate while 5,6-LAM can accept D-lysine and L-β-lysine. This review focuses on recent computational, spectroscopic and structural studies of these enzymes and their implications on the related enzymes. Additionally, we also discuss the potential biosynthetic application of 5,6-LAM. View Full-Text
Keywords: coenzyme B12 (5'-deoxyadenosylcobalamin; dAdoCbl); pyridoxal-5'-phosphate (PLP; vitamin B6); lysine 5,6-aminomutase; ornithine 4,5-aminomutase; isotope-edited electron paramagnetic resonance (EPR) and electron-nuclear double resonance (ENDOR) spectroscopy; density functional theory (DFT) coenzyme B12 (5'-deoxyadenosylcobalamin; dAdoCbl); pyridoxal-5'-phosphate (PLP; vitamin B6); lysine 5,6-aminomutase; ornithine 4,5-aminomutase; isotope-edited electron paramagnetic resonance (EPR) and electron-nuclear double resonance (ENDOR) spectroscopy; density functional theory (DFT)
MDPI and ACS Style

Maity, A.N.; Chen, Y.-H.; Ke, S.-C. Large-Scale Domain Motions and Pyridoxal-5'-Phosphate Assisted Radical Catalysis in Coenzyme B12-Dependent Aminomutases. Int. J. Mol. Sci. 2014, 15, 3064-3087.

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