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Expression of the MT1 Melatonin Receptor in Ovarian Cancer Cells

Department of Histology and Embryology, Wrocław Medical University, Wrocław 50-368, Poland
Department of Physiotherapy, University School of Physical Education, Wrocław 51-612, Poland
Department of Gynecology, University of Medical Sciences, Poznań 60-535, Poland
Department of Gynecological Oncology, University of Medical Sciences, Poznań 60-535, Poland
Department of Histology and Embryology, University of Medical Sciences, Poznań 60-781, Poland
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(12), 23074-23089;
Received: 14 October 2014 / Revised: 20 November 2014 / Accepted: 21 November 2014 / Published: 12 December 2014
(This article belongs to the Special Issue Advances in the Research of Melatonin 2014)
PDF [1562 KB, uploaded 12 December 2014]


Ovarian cancer (OC) is the leading cause of death among women with genital tract disorders. Melatonin exhibits oncostatic properties which it may effect through binding to its membrane receptor, MT1. The aim of this study was to determine the expression of MT1 in OC cells and to correlate this with clinical and pathological data. Immunohistochemistry was performed on 84 cases of OC. Normal ovarian epithelial (IOSE 364) and OC (SK-OV-3, OVCAR-3) cell lines were used to examine the MT1 expression at protein level using the western blot and immunofluorescence technique. The expression of MT1 was observed as cytoplasmic-membrane (MT1CM) and membrane (MT1M) reactions. A positive correlation between MT1CM and MT1M was found in all the studied cases. There were no significant differences between the expression of MT1CM, MT1M, and histological type, staging, grading, presence of residual disease, or overall survival time. Immunofluorescence showed both MT1M and MT1CM expression in all the tested cell lines. Western blot illustrated the highest protein level of MT1 in IOSE 364 and the lowest in the OVCAR-3. The results indicate the limited prognostic significance of MT1 in OC cells. View Full-Text
Keywords: melatonin; melatonin receptor; ovarian cancer melatonin; melatonin receptor; ovarian cancer

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Jablonska, K.; Pula, B.; Zemla, A.; Kobierzycki, C.; Kedzia, W.; Nowak-Markwitz, E.; Spaczynski, M.; Zabel, M.; Podhorska-Okolow, M.; Dziegiel, P. Expression of the MT1 Melatonin Receptor in Ovarian Cancer Cells. Int. J. Mol. Sci. 2014, 15, 23074-23089.

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