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Int. J. Mol. Sci. 2014, 15(12), 22772-22785;

Regulated Necrosis in HeLa Cells Induced by ZnPc Photodynamic Treatment: A New Nuclear Morphology

Department of Biology, University Autonomous of Madrid, 28049 Madrid, Spain
Author to whom correspondence should be addressed.
Received: 7 October 2014 / Revised: 3 November 2014 / Accepted: 1 December 2014 / Published: 9 December 2014
(This article belongs to the Section Biochemistry)
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Photodynamic therapy (PDT) is a cancer treatment modality based on the administration of a photosensitizer (PS), which accumulates preferentially in tumor cells. Subsequent irradiation of the neoplastic area triggers a cascade of photochemical reactions that leads to the formation of highly reactive oxygen species responsible for cell inactivation. Photodynamic treatments in vitro are performed with the PS, zinc-phthalocyanine (ZnPc). The PS is near the plasma membrane during uptake and internalization. Inactivation clearly occurs by a necrotic process, manifested by nuclear pyknosis, negative TUNEL and Annexin V assays and non-relocation of cytochrome c. In contrast, by increasing the incubation time, ZnPc is accumulated in the Golgi apparatus and produces cell inactivation with characteristics of apoptosis and necrosis: TUNEL positive, relocated cytochrome c and negative Annexin V assay. This type of death produces a still undescribed granulated nuclear morphology, which is different from that of necrosis or apoptosis. This morphology is inhibited by necrostatin-1, a specific inhibitor of regulated necrosis. View Full-Text
Keywords: photodynamic therapy; photosensitizer; apoptosis; necrosis; regulated necrosis photodynamic therapy; photosensitizer; apoptosis; necrosis; regulated necrosis

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Soriano, J.; Villanueva, A.; Stockert, J.C.; Cañete, M. Regulated Necrosis in HeLa Cells Induced by ZnPc Photodynamic Treatment: A New Nuclear Morphology. Int. J. Mol. Sci. 2014, 15, 22772-22785.

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