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Open AccessArticle

The LBP Gene and Its Association with Resistance to Aeromonas hydrophila in Tilapia

by Gui Hong Fu 1,2, Feng Liu 1, Jun Hong Xia 1,3 and Gen Hua Yue 1,4,5,*
Molecular Population Genetics & Breeding Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore
Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Shanghai 200090, China
School of Life Sciences, Sun Yat-Sen University, Guangzhou 266061, China
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(12), 22028-22041;
Received: 2 October 2014 / Revised: 17 November 2014 / Accepted: 24 November 2014 / Published: 1 December 2014
(This article belongs to the Section Biochemistry)
Resistance to pathogens is important for the sustainability and profitability of food fish production. In immune-related genes, the lipopolysaccharide-binding protein (LBP) gene is an important mediator of the inflammatory reaction. We analyzed the cDNA and genomic structure of the LBP gene in tilapia. The full-length cDNA (1901 bp) of the gene contained a 1416 bp open reading frame, encoding 471 amino acid residues. Its genomic sequence was 5577 bp, comprising 15 exons and 14 introns. Under normal conditions, the gene was constitutively expressed in all examined tissues. The highest expression was detected in intestine and kidney. We examined the responses of the gene to challenges with two bacterial pathogens Streptcoccus agalactiae and Aeromonas hydrophila. The gene was significantly upregulated in kidney and spleen post-infection with S. agalactiae and A. hydrophila, respectively. However, the expression profiles of the gene after the challenge with the two pathogens were different. Furthermore, we identified three SNPs in the gene. There were significant associations (p < 0.05) of two of the three SNPs with the resistance to A. hydrophila, but not with the resistance to S. agalactiae or growth performance. These results suggest that the LBP gene is involved in the acute-phase immunologic response to the bacterial infections, and the responses to the two bacterial pathogens are different. The two SNPs associated with the resistance to A. hydrophila may be useful in the selection of tilapia resistant to A. hydrophila. View Full-Text
Keywords: tilapia; gene; SNP; pathogen; association tilapia; gene; SNP; pathogen; association
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Fu, G.H.; Liu, F.; Xia, J.H.; Yue, G.H. The LBP Gene and Its Association with Resistance to Aeromonas hydrophila in Tilapia. Int. J. Mol. Sci. 2014, 15, 22028-22041.

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