Discovery and in Vivo Evaluation of Novel RGD-Modified Lipid-Polymer Hybrid Nanoparticles for Targeted Drug Delivery
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Curcumin (Cur)–Lipid-Shell and Polymer Core Nanoparticles (lpNPs)
Drug/Polymer (%, w/w) | Size (nm) | Polydispersity Index (PDI) | DL (%) | EE (%) |
---|---|---|---|---|
2 | 201.9 ± 7.8 | 0.195 ± 0.05 | 2.0 ± 0.01 | 100 ± 0.24 |
3 | 206.36 ± 6.1 | 0.192 ± 0.07 | 2.96 ± 0.02 | 98.67 ± 0.67 |
4 | 202.4 ± 5.2 | 0.202 ± 0.03 | 3.91 ± 0.02 | 97.75 ± 0.5 |
5 | 216.6 ± 4.7 | 0.205 ± 0.02 | 4.80 ± 0.03 | 96.0 ± 0.6 |
7 | 260.5 ± 8.1 | 0.282 ± 0.07 | 4.95 ± 0.07 | 70.71 ± 1.0 |
2.2. In Vitro Cytotoxicity Evaluation
2.3. In Vitro Apoptosis Induction Effect
2.4. Cellular Uptake of Cur–lpNPs by Flow Cytometry Analysis
2.5. In Vivo Anti-Tumor Effect in Subcutaneous Tumor Model
2.6. Determination of Apoptosis
2.7. Quantitative Assessment of Microvessel Density (MVD)
3. Experimental Section
3.1. Materials, Cell Lines and Animals
3.2. Preparation and Characterization of Cur–lpNPs
3.3. In Vitro Cytotoxicity
3.4. Cellular Apoptosis Assay
3.5. Cellular Uptake of Cur–lpNPS by Flow Cytometry Analysis
3.6. Subcutaneous Tumor Mouse Model
3.7. Detection of Apoptosis
3.8. Quantitative Assessment of MVD
3.9. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
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Zhao, Y.; Lin, D.; Wu, F.; Guo, L.; He, G.; Ouyang, L.; Song, X.; Huang, W.; Li, X. Discovery and in Vivo Evaluation of Novel RGD-Modified Lipid-Polymer Hybrid Nanoparticles for Targeted Drug Delivery. Int. J. Mol. Sci. 2014, 15, 17565-17576. https://doi.org/10.3390/ijms151017565
Zhao Y, Lin D, Wu F, Guo L, He G, Ouyang L, Song X, Huang W, Li X. Discovery and in Vivo Evaluation of Novel RGD-Modified Lipid-Polymer Hybrid Nanoparticles for Targeted Drug Delivery. International Journal of Molecular Sciences. 2014; 15(10):17565-17576. https://doi.org/10.3390/ijms151017565
Chicago/Turabian StyleZhao, Yinbo, Dayong Lin, Fengbo Wu, Li Guo, Gu He, Liang Ouyang, Xiangrong Song, Wei Huang, and Xiang Li. 2014. "Discovery and in Vivo Evaluation of Novel RGD-Modified Lipid-Polymer Hybrid Nanoparticles for Targeted Drug Delivery" International Journal of Molecular Sciences 15, no. 10: 17565-17576. https://doi.org/10.3390/ijms151017565