MiRNA-199a-3p Regulates C2C12 Myoblast Differentiation through IGF-1/AKT/mTOR Signal Pathway
Abstract
:1. Introduction
2. Results
2.1. Tissue Expression Profile of MiR-199a-3p and Its Expression Pattern during C2C12 Myogenic Differentiation
2.2. Analysis of MiR-199a-3p Target Genes
2.3. Over-Expression of MiR-199a-3p Inhibits C2C12 Myogenic Differentiation
2.4. Inhibition of MiR-199a-3p Promotes Myogenic Differentiation and Myotube Hypertrophy
2.5. MiR-199a-3p Affects IGF/AKT/mTOR Pathway in C2C12 Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Transfection of MiR-199a-3p Mimic and Inhibitor
4.3. Immunocytochemical Analysis
4.4. Real-Time qPCR
4.5. Western Blot Detection
4.6. Luciferase Reporter Assay
4.7. Statistic Analysis
5. Conclusions
Supplementary Information
ijms-15-00296-s001.pdfAcknowledgments
Conflicts of Interest
References
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Jia, L.; Li, Y.-F.; Wu, G.-F.; Song, Z.-Y.; Lu, H.-Z.; Song, C.-C.; Zhang, Q.-L.; Zhu, J.-Y.; Yang, G.-S.; Shi, X.-E. MiRNA-199a-3p Regulates C2C12 Myoblast Differentiation through IGF-1/AKT/mTOR Signal Pathway. Int. J. Mol. Sci. 2014, 15, 296-308. https://doi.org/10.3390/ijms15010296
Jia L, Li Y-F, Wu G-F, Song Z-Y, Lu H-Z, Song C-C, Zhang Q-L, Zhu J-Y, Yang G-S, Shi X-E. MiRNA-199a-3p Regulates C2C12 Myoblast Differentiation through IGF-1/AKT/mTOR Signal Pathway. International Journal of Molecular Sciences. 2014; 15(1):296-308. https://doi.org/10.3390/ijms15010296
Chicago/Turabian StyleJia, Long, Yue-Feng Li, Guo-Fang Wu, Zi-Yi Song, Hong-Zhao Lu, Cheng-Chuang Song, Qiang-Ling Zhang, Jia-Yu Zhu, Gong-She Yang, and Xin-E Shi. 2014. "MiRNA-199a-3p Regulates C2C12 Myoblast Differentiation through IGF-1/AKT/mTOR Signal Pathway" International Journal of Molecular Sciences 15, no. 1: 296-308. https://doi.org/10.3390/ijms15010296