Next Article in Journal
Formation of Apatite Coatings on an Artificial Ligament Using a Plasma- and Precursor-Assisted Biomimetic Process
Next Article in Special Issue
Carcinogenesis of Pancreatic Adenocarcinoma: Precursor Lesions
Previous Article in Journal
Small Molecules Present in the Cerebrospinal Fluid Metabolome Influence Superoxide Dismutase 1 Aggregation
Previous Article in Special Issue
Emerging Roles of Claudins in Human Cancer
Open AccessArticle

Probing Antigen-Antibody Interaction Using Fluorescence Coupled Capillary Electrophoresis

by Jianhao Wang 1,†, Lin Qiu 1,†, Cheli Wang 1, Yue Zhang 1, Jingyan Li 1, Jiang Xia 2,* and Pengju Jiang 1,*
School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China
Department of Chemistry, the Chinese University of Hong Kong, Shatin, Hong Kong
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2013, 14(9), 19146-19154;
Received: 23 August 2013 / Revised: 4 September 2013 / Accepted: 5 September 2013 / Published: 17 September 2013
(This article belongs to the Special Issue Molecular Bases of Cancer Research)
In this report, the use of fluorescence detection coupled capillary electrophoresis (CE-FL) allowed us to fully characterize the antigen-antibody interaction. CE-FL allowed separation of unbound quantum dots (QDs) and ligand bound QDs and also revealed an ordered assembly of biomolecules on QDs. Further, we observed FRET from QDs donor to DyLight acceptor, which were covalently conjugated with human IgG and goat anti-human IgG, respectively. The immunocomplex was formed and the mutual affinity of the antigen and antibody brought QDs and DyLight close enough to allow FRET to occur. This novel CE-based technique can be easily extended to other FRET systems based on QDs and may have potential application in the detection of antibodies. View Full-Text
Keywords: QDs; FRET; immunocomplex; capillary electrophoresis QDs; FRET; immunocomplex; capillary electrophoresis
Show Figures

Graphical abstract

MDPI and ACS Style

Wang, J.; Qiu, L.; Wang, C.; Zhang, Y.; Li, J.; Xia, J.; Jiang, P. Probing Antigen-Antibody Interaction Using Fluorescence Coupled Capillary Electrophoresis. Int. J. Mol. Sci. 2013, 14, 19146-19154.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.
Search more from Scilit
Back to TopTop